TY - GEN T1 - Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti - identification of stage-specific proteases. AU - Soblik, Hanns AU - Younis, Abuelhassan Elshazly AU - Mitreva, Makedonka AU - Renard, Bernhard Y. AU - Kirchner, Marc AU - Geisinger, Frank AU - Steen, Hanno AU - Brattig, Norbert W. AB - A wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. Excretory and secretory proteins (ESP) are active at the interface between parasite and host and comprise potential targets for intervention. The intestinal nematode Strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living generations. We investigated ESP from infective larvae, parasitic females, and free-living stages of the rat parasite Strongyloides ratti, which is genetically very similar to the human pathogen, Strongyloides stercoralis. Proteomic analysis of ESP revealed 586 proteins, with the largest number of stage-specific ESP found in infective larvae (196), followed by parasitic females (79) and free-living stages (35). One hundred and forty proteins were identified in all studied stages, including anti-oxidative enzymes, heat shock proteins, and carbohydrate-binding proteins. The stage-selective ESP of (1) infective larvae included an astacin metalloproteinase, the L3 Nie antigen, and a fatty acid retinoid-binding protein; (2) parasitic females included a prolyl oligopeptidase (prolyl serine carboxypeptidase), small heat shock proteins, and a secreted acidic protein; (3) free-living stages included a lysozyme family member, a carbohydrate-hydrolyzing enzyme, and saponin-like protein. We verified the differential expression of selected genes encoding ESP by qRT-PCR. ELISA analysis revealed the recognition of ESP by antibodies of S. ratti-infected rats. A prolyl oligopeptidase was identified as abundant parasitic female-specific ESP, and the effect of pyrrolidine-based prolyl oligopeptidase inhibitors showed concentration- and time-dependent inhibitory effects on female motility. The characterization of stage-related ESP from Strongyloides will help to further understand the interaction of this unique intestinal nematode with its host. KW - Amino Acid Sequence KW - Gene Expression Regulation KW - Molecular Sequence Data KW - Female KW - Male KW - Animals KW - Base Sequence KW - Rats KW - Protease Inhibitors/pharmacology KW - Proteomics KW - Culture Media/chemistry KW - Developmental KW - Helminth Proteins/genetics KW - Helminth Proteins/metabolism KW - Helminth Proteins/secretion KW - Immune Sera/chemistry KW - Intestines/parasitology KW - Larva/enzymology KW - Larva/genetics KW - Larva/growth & development KW - Peptide Hydrolases/genetics KW - Peptide Hydrolases/metabolism KW - Peptide Hydrolases/secretion KW - Protein Sorting Signals KW - Protein Structure Tertiary KW - Rats Wistar KW - Real-Time Polymerase Chain Reaction KW - Sequence Analysis Protein KW - Serine Endopeptidases/genetics KW - Serine Endopeptidases/metabolism KW - Serine Endopeptidases/secretion KW - Statistics Nonparametric KW - Strongyloides ratti/enzymology KW - Strongyloides ratti/genetics KW - Strongyloides ratti/growth & development KW - Strongyloidiasis/parasitology KW - 610 Medizin PY - 2011 LA - eng PB - Robert Koch-Institut VL - 10 IS - 12 DO - 10.1074/mcp.M111.010157 ER -