TY - GEN T1 - Typing of Salmonella enterica serovar Infantis isolates from 51 outbreaks in Germany between 1974 and 2009 by a novel phage-typing scheme AU - Miller, Tatjana AU - Braun, Peggy AU - Fehlhaber, K. AU - Prager, Rita AU - Pfeifer, Yvonne AU - Rabsch, Wolfgang AB - We developed a new phage-typing method and evaluated its application in combination with XbaI macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) as a useful tool for the long-term epidemiology of Salmonella enterica serovar Infantis. In this study, we investigated 1008 S. Infantis isolates recovered from humans, various animal species and food products from 1973 to 2009. The typing scheme is based on 17 typing phages, defining 61 different patterns within the strain collection. The experiments showed that phage typing is a reliable method for differentiation of outbreaks and sporadic clinical cases as well as for elucidation of chains of transmission. The combined analysis of phage typing and PFGE revealed the existence of epidemic clones with a high stability over time like PT29/XB27 which was identified in nosocomial salmonellosis, community outbreaks as well as in broiler chickens from 2002 to 2009. KW - Animals KW - Humans KW - Germany/epidemiology KW - Cattle KW - Disease Outbreaks KW - Anti-Bacterial Agents/pharmacology KW - Bacteriophage Typing/methods KW - Chickens KW - Dogs KW - Drug Resistance Bacterial KW - Electrophoresis Gel Pulsed-Field KW - Food Microbiology KW - Meat/microbiology KW - Microbial Sensitivity Tests KW - Salmonella Infections/epidemiology KW - Salmonella Infections/microbiology KW - Salmonella enterica/classification KW - Salmonella enterica/drug effects KW - Salmonella enterica/isolation & purification KW - Salmonella enterica/virology KW - 610 Medizin PY - 2013 LA - eng PB - Robert Koch-Institut, Infektionskrankheiten / Erreger VL - 142 IS - 1 DO - 10.1017/S095026881300037X ER -