TY - GEN T1 - Cross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa AU - Bielecki, Piotr AU - Jensen, Vanessa AU - Schulze, Wiebke AU - Gödeke, Julia AU - Strehmel, Janine AU - Eckweiler, Denitsa AU - Nicolai, Tanja AU - Bielecka, Agata AU - Wille, Thorsten AU - Gerlach, Roman AU - Häussler, Susanne AB - Two-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 TCS components. To avoid unwanted cross-talk, signaling cascades are very specific, with one sensor talking to its cognate response regulator (RR). However, cross-regulation may provide means to integrate different environmental stimuli into a harmonized output response. By applying a split luciferase complementation assay, we identified a functional interaction of two RRs of the OmpR/PhoB subfamily, namely PhoB and TctD in P. aeruginosa. Transcriptional profiling, ChIP-seq analysis and a global motif scan uncovered the regulons of the two RRs as well as a quadripartite binding motif in six promoter regions. Phosphate limitation resulted in PhoB-dependent expression of the downstream genes, whereas the presence of TctD counteracted this activation. Thus, the integration of two important environmental signals e.g. phosphate availability and the carbon source are achieved by a titration of the relative amounts of two phosphorylated RRs that inversely regulate a common subset of genes. In conclusion, our results on the PhoB and TctD mediated two-component signal transduction pathways exemplify how P. aeruginosa may exploit cross-regulation to adapt bacterial behavior to complex environments. KW - Bacterial KW - Bacterial Proteins/metabolism KW - DNA-Binding Proteins/genetics KW - DNA-Binding Proteins/metabolism KW - Bacterial Proteins/genetics KW - Base Sequence KW - Signal Transduction KW - Transcription Genetic KW - Luciferases/genetics KW - Binding Sites KW - Consensus Sequence KW - Gene Expression Regulation KW - Luciferases/analysis KW - Promoter Regions Genetic KW - Pseudomonas aeruginosa/genetics KW - Pseudomonas aeruginosa/metabolism KW - Regulon KW - 610 Medizin PY - 2015 LA - eng PB - Robert Koch-Institut VL - 43 IS - 13 DO - 10.1093/nar/gkv599 ER -