TY - GEN T1 - Comparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the Species- and Strain-Specific Proteome AU - Döllinger, Jörg AU - Schaade, Lars AU - Nitsche, Andreas AB - Cowpox virus (CPXV) causes most zoonotic orthopoxvirus (OPV) infections in Europe and Northern as well as Central Asia. The virus has the broadest host range of OPV and is transmitted to humans from rodents and other wild or domestic animals. Increasing numbers of human CPXV infections in a population with declining immunity have raised concerns about the virus’ zoonotic potential. While there have been reports on the proteome of other human-pathogenic OPV, namely vaccinia virus (VACV) and monkeypox virus (MPXV), the protein composition of the CPXV mature virion (MV) is unknown. This study focused on the comparative analysis of the VACV and CPXV MV proteome by label-free single-run proteomics using nano liquid chromatography and high-resolution tandem mass spectrometry (nLC-MS/MS). The presented data reveal that the common VACV and CPXV MV proteome contains most of the known conserved and essential OPV proteins and is associated with cellular proteins known to be essential for viral replication. While the species-specific proteome could be linked mainly to less genetically-conserved gene products, the strain-specific protein abundance was found to be of high variance in proteins associated with entry, host-virus interaction and protein processing. KW - Species Specificity KW - Viral Proteins/genetics KW - Capsid Proteins/genetics KW - Virus Replication KW - Genes Viral KW - DNA Viral/genetics KW - Proteome KW - Capsid Proteins/analysis KW - Chromatography Liquid/methods KW - Cowpox virus/chemistry KW - Cowpox virus/genetics KW - Cowpox virus/physiology KW - Nanotechnology/methods KW - Tandem Mass Spectrometry/methods KW - Vaccinia virus/chemistry KW - Vaccinia virus/genetics KW - Vaccinia virus/physiology KW - Viral Plaque Assay KW - Viral Proteins/analysis KW - Virion/chemistry KW - Virion/isolation & purification KW - 610 Medizin PY - 2015 LA - eng PB - Robert Koch-Institut, Biologische Sicherheit VL - 10 IS - 11 DO - 10.1371/journal.pone.0141527 ER -