TY - JOUR T1 - Detection and Characterization of Human Enteroviruses, Human Cosaviruses, and a New Human Parechovirus Type in Healthy Individuals in Osun State, Nigeria, 2016/2017 AU - Abiodun Osundare, Folakemi AU - Oluyinka Opaleye, Oladele AU - Abiodun Akindele, Akeem AU - Adeyinka Adedokun, Samuel AU - Anuoluwapo Akanbi, Olusola AU - Bock, Claus-Thomas AU - Diedrich, Sabine AU - Böttcher, Sindy AB - Human enteroviruses and human parechoviruses are associated with a broad range of diseases and even severe and fatal conditions. For human cosaviruses, the etiological role is yet unknown. Little is known about the circulation of non-polio enteroviruses, human parechoviruses, and human cosaviruses in Nigeria. A total of 113 stool samples were collected from healthy individuals in Osun State between February 2016 and May 2017. RT-PCR assays targeting the 5′ non-coding region (5′ -NCR) were used to screen for human enteroviruses, human parechoviruses, and human cosaviruses. For human enteroviruses, species-specific RT-PCR assays targeting the VP1 regions were used for molecular typing. Inoculation was carried out on RD-A, CaCo-2, HEp-2C, and L20B cell lines to compare molecular and virological assays. Ten samples tested positive for enterovirus RNA with 11 strains detected, including CV-A13 (n = 3), E-18 (n = 2), CV-A20 (n = 1), CV-A24 (n = 1), EV-C99 (n = 1), and EV-C116 (n = 2). Three samples tested positive for human parechovirus RNA, and full genome sequencing on two samples allowed assignment to a new Parechovirus A type (HPeV-19). Thirty-three samples tested positive for cosavirus with assignment to species Cosavirus D and Cosavirus A based on the 5′-NCR region. Screening of stool samples collected from healthy individuals in Nigeria in 2016 and 2017 revealed a high diversity of circulating human enteroviruses, human parechoviruses, and human cosaviruses. Molecular assays for genotyping showed substantial benefits compared with those of cell-culture assays. KW - enteroviruses KW - parechoviruses KW - cosaviruses KW - poliovirus KW - containment KW - Nigeria KW - picornavirus KW - 610 Medizin und Gesundheit PY - 2019 LA - eng PB - Robert Koch-Institut JO - Viruses VL - 11 IS - 1037 SP - 1 EP - 12 DO - 10.3390/v11111037 ER -