TY - GEN T1 - Rapid detection of anti-Vaccinia virus neutralizing antibodies AU - Kramski, Marit AU - Drozd, Anna AU - Lichtfuss, Gregor F. AU - Dabrowski, Piotr Wojtek AU - Ellerbrok, Heinz AB - Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR. KW - Cell Line KW - Humans KW - Virus Replication KW - Polymerase Chain Reaction/methods KW - Vaccinia virus/isolation & purification KW - Neutralization Tests KW - Vaccinia virus/genetics KW - Antibodies Viral/immunology KW - Antibodies Neutralizing/analysis KW - Antibodies Neutralizing/immunology KW - Antibodies Viral/analysis KW - Vaccinia/diagnosis KW - Vaccinia/immunology KW - Vaccinia virus/immunology KW - Vaccinia virus/physiology KW - 610 Medizin PY - 2011 LA - eng PB - Robert Koch-Institut, Biologische Sicherheit VL - 8 IS - 139 DO - 10.1186/1743-422X-8-139 ER -