TY - GEN T1 - No Detection of XMRV in Blood Samples and Tissue Sections from Prostate Cancer Patients in Northern Europe. AU - Stieler, Kristin AU - Schindler, Sarah AU - Schlomm, Thorsten AU - Hohn, Oliver AU - Bannert, Norbert AU - Simon, Ronald AU - Minner, Sarah AU - Schindler, Michael AU - Fischer, Nicole AB - Background: We recently published the rare detection of xenotropic murine leukemia virus-related virus (XMRV) (1/105) in prostate cancer (PCA) tissue of patients in Northern Europe by PCR. The controversial discussion about the virus being detected in PCA tissue, blood samples from patients suffering from chronic fatigue syndrome (CFS), as well as from a significant number of healthy controls prompted us to deepen our studies about detection of XMRV infection applying different detection methods (PCR, cocultivation and immunohistochemistry [IHC]). Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMCs) from 92 PCA and 7 healthy controls were isolated, PHA activated and cocultivated with LNCaP cells for up to 8 weeks. Supernatant of these cells was applied to a reporter cell line, DERSE-iGFP. Furthermore, the PBMCs and cocultivated LNCaP cells were tested for the presence of XMRV by PCR as well as Western Blot analysis. While all PCR amplifications and Western Blot analyses were negative for signs of XMRV infection, DERSE-iGFP cells displayed isolated GFP positive cells in three cases. In all three cases XMRV presence could not be confirmed by PCR technology. In addition, we performed XMRV specific IHC on PCA tissue sections. Whole tissue sections (n = 20), as well as tissue microarrays (TMA) including 50 benign prostate hyperplasia (BPH), 50 low grade and 50 high grade PCA sections and TMAs including breast cancer, colon cancer and normal tissues were stained with two XMRV specific antisera. XMRV protein expression was not detected in any cancer sections included. One BPH tissue displayed XMRV specific protein expression in random isolated basal cells. Conclusion: We were unable to conclusively detect XMRV in the blood from PCA patients or from healthy controls and there is no conclusive evidence of XMRV protein expression in PCA, breast cancer and colon cancer tissue sections tested by IHC staining. KW - Adult KW - Humans KW - Polymerase Chain Reaction KW - Male KW - Middle Aged KW - Viral Proteins/metabolism KW - Virus Replication KW - Case-Control Studies KW - Aged KW - Europe KW - Prostatic Neoplasms/virology KW - Retroviridae Infections/virology KW - Cell Line Tumor KW - Leukocytes Mononuclear/virology KW - Xenotropic murine leukemia virus-related virus/genetics KW - Cell Separation KW - Coculture Techniques KW - Immunohistochemistry KW - Lymphocyte Activation/immunology KW - Prostate/pathology KW - Prostate/virology KW - Prostatic Neoplasms/blood KW - Prostatic Neoplasms/pathology KW - Xenotropic murine leukemia virus-related virus/isolation & purification KW - Xenotropic murine leukemia virus-related virus/physiology KW - 610 Medizin PY - 2011 LA - eng PB - Robert Koch-Institut, Infektionsepidemiologie VL - 6 IS - 10 DO - 10.1371/journal.pone.0025592 ER -