http://edoc.rki.de/176904/44 Wed, 22 Apr 2026 10:45:54 GMT 2026-04-22T10:45:54Z http://edoc.rki.de/176904/13644 Genotypic Characterization of Uropathogenic Escherichia coli from Companion Animals: Predominance of ST372 in Dogs and Human-Related ST73 in Cats Aurich, Sophie; Wolf, Silver Anthony; Prenger-Berninghoff, Ellen; Thrukonda, Lakshmipriya; Semmler, Torsten; Ewers, Christa Extraintestinal pathogenic Escherichia coli (ExPEC) account for over 80% and 60% of bacterial urinary tract infections (UTIs) in humans and animals, respectively. As shared uropathogenic E. coli (UPEC) strains have been previously reported among humans and pets, our study aimed to characterize E. coli lineages among UTI isolates from dogs and cats and to assess their overlaps with human UPEC lineages. We analysed 315 non-duplicate E. coli isolates from the UT of dogs (198) and cats (117) collected in central Germany in 2019 and 2020 utilizing whole genome sequencing and in silico methods. Phylogroup B2 (77.8%), dog-associated sequence type (ST) 372 (18.1%), and human-associated ST73 (16.6%), were predominant. Other STs included ST12 (8.6%), ST141 (5.1%), ST127 (4.8%), and ST131 (3.5%). Among these, 58.4% were assigned to the ExPEC group and 51.1% to the UPEC group based on their virulence associated gene (VAG) profile (ExPEC, presence of ≥VAGs: papAH and/or papC, sfa/focG, afaD/draBC, kpsMTII, and iutA; UPEC, additionally cnf1 or hlyD). Extended-spectrum cephalosporin (ESC) resistance mediated by extended-spectrum β-lactamases (ESBL) and AmpC-β-lactamase was identified in 1.9% of the isolates, along with one carbapenemase-producing isolate and one isolate carrying a mcr gene. Low occurrence of ESC-resistant or multidrug-resistant (MDR) isolates (2.9%) in the two most frequently detected STs implies that E. coli isolated from UTIs of companion animals are to a lesser extent associated with resistance, but possess virulence-associated genes enabling efficient UT colonization and carriage. Detection of human-related pandemic lineages suggests interspecies transmission and underscores the importance of monitoring companion animals. Sat, 30 Dec 2023 00:00:00 GMT http://edoc.rki.de/176904/13644 2023-12-30T00:00:00Z http://edoc.rki.de/176904/13643 Burden and epidemiology of Campylobacter species in acute enteritis cases in Burkina Faso Badjo, Ange Oho Roseline; Kabore, Nongodo Firmin; Zongo, Arsène; Gnada, Kobo; Ouattara, Aminata; Muhigwa, Merci; Ouangraoua, Soumeya; Poda, Armel; Some, Satouro Arsène; Schubert, Grit; Eckmanns, Tim; Leendertz, Fabian H.; Belarbi, Essia; Ouedraogo, Abdoul-Salam Background: Campylobacter spp. is a significant etiological agent of bacterial gastroenteritis globally. In Burkina Faso (BFA), the actual impact of this pathogen on gastroenteritis is considerably underestimated, primarily due to inadequate surveillance systems. Objectives: This study aimed to investigate the proportion of Campylobacter species responsible for acute gastroenteritis among patients of all ages in urban and rural areas of BFA, using molecular biology techniques. Study design & methods: Between 2018 and 2021, faecal specimens were obtained from 1,295 individuals presenting with acute gastroenteritis. These samples underwent screening for the Campylobacter coli/jejuni/lari complex utilizing real-time polymerase chain reaction (PCR) assays. Subsequently, positive samples were subjected to species-level differentiation through the application of species-specific primers. Results: Campylobacter spp. was detected in 25.0% (324/1,295) of the samples analysed. The majority of positive samples (95%, 308/324) were obtained from children under 5 years of age. Species identification was performed on a subset of 114 isolates, revealing 51 Campylobacter jejuni, 10 Campylobacter coli, and 53 Campylobacter isolates that remained unspeciated. Conclusions: This study reveals a significant prevalence of Campylobacter species among patients with acute gastroenteritis, with a particularly high incidence observed in children under 5 years of age. Based on these findings, the implementation of routine Campylobacter surveillance in public health laboratories is strongly recommended to better monitor and address this health concern. Fri, 09 Aug 2024 00:00:00 GMT http://edoc.rki.de/176904/13643 2024-08-09T00:00:00Z http://edoc.rki.de/176904/13642 Physical Activity-Related Health Competence Among Adults Jordan, S.; Domanska, O. M.; Carl, J.; Manz, K.; Buchmann, M.; Loer, A. K. M.; Pfeifer, K.; Sudeck, G. Mon, 17 Jun 2024 00:00:00 GMT http://edoc.rki.de/176904/13642 2024-06-17T00:00:00Z http://edoc.rki.de/176904/13641 The Percentage of Antibiotic Resistance in Uncomplicated Community-Acquired Urinary Tract Infections Klingeberg, Anja; Willrich, Niklas; Schneider, Marc; Schmiemann, Guido; Gágyor, Ildikó; Richter, Doreen; Noll, Ines; Eckmanns, Tim Background: Uncomplicated bacterial urinary tract infections (uUTIs) are commonly seen in outpatient practice. They are usually treated empirically with antibiotics. The pertinent German Clinical Practice Guideline contains recommendations on antibiotic selection, with the additional advice that the local resistance situation should be considered as well. However, up-to-date information on local resistance is often unavailable, because microbiological testing is mainly recommended for complicated UTIs. Resistance rates are often higher in recurrent uUTIs than in single episodes. In this study, we aimed to determine the resistance rates of Escherichia coli (E. coli) in patients with community-acquired uUTIs and to make these data available to the treating physicians. Methods: In a nationwide cross-sectional study in Germany (DRKS 00019059), we determined the percentages of resistance to antibiotics recommended for uUTIs (first choice: fosfomycin, nitroxoline, mecillinam, nitrofurantoin, trimethoprim; second choice: cefpodoxime, ciprofloxacin, cotrimoxazole, levofloxacin, norfloxacin, ofloxacin) over the period 2019–2021. The data were stratified by single episodes vs. recurrent UTIs (rUTIs). Results: Data from 2390 subjects were analyzed. E. coli was found in 75.4% of the samples with positive urine cultures (1082 out of 1435). The resistance rate of E. coli in single episodes (n = 725) was less than 15% for all antibiotics tested. In rUTIs (n = 357), resistance rates were also less than 15% for the most part; the only exceptions were trimethoprim (21.4%) and cotrimoxazole (19.3%). Conclusion: For single episodes of uUTI, all of the antibiotics studied can be recommended, at least as far as their resistance profiles are concerned. For recurrent UTI, all but trimethoprim and cotrimoxazole can be recommended. The second-choice antibiotics examined do not have a more favorable resistance profile than the first-choice antibiotics. Tue, 05 Dec 2023 00:00:00 GMT http://edoc.rki.de/176904/13641 2023-12-05T00:00:00Z