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2022-03-31Zeitschriftenartikel
No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020
dc.contributor.authorFaber, Mirko
dc.contributor.authorWenzel, Jürgen J.
dc.contributor.authorErl, Monika
dc.contributor.authorStark, Klaus
dc.contributor.authorSchemmerer, Mathias
dc.date.accessioned2022-08-30T06:47:02Z
dc.date.available2022-08-30T06:47:02Z
dc.date.issued2022-03-31none
dc.identifier.other10.3390/ v14040742
dc.identifier.urihttp://edoc.rki.de/176904/10104
dc.description.abstractOrthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%–1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjecthepatitis E viruseng
dc.subjectorthohepevirus Ceng
dc.subjectrat HEVeng
dc.subjectepidemiologyeng
dc.subjectphylogenyeng
dc.subjectGermanyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleNo Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000–2020none
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/10104-9
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.container-issn1999-4915none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/14/4/742/htmnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume14none
local.edoc.container-issue4none
local.edoc.container-year2022none
dc.description.versionPeer Reviewednone

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