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2011-12-27Zeitschriftenartikel DOI: 10.1371/journal.pntd.0001406
Single Dose Novel Salmonella Vaccine Enhances Resistance against Visceralizing L. major and L. donovani Infection in Susceptible BALB/c Mice.
dc.contributor.authorSchroeder, Juliane
dc.contributor.authorBrown, Najmeeyah
dc.contributor.authorKaye, Paul
dc.contributor.authorAebischer, Toni
dc.date.accessioned2018-05-07T15:06:53Z
dc.date.available2018-05-07T15:06:53Z
dc.date.created2012-01-10
dc.date.issued2011-12-27none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/repspttgc0w/PDF/27j4jDHkseZc.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1041
dc.description.abstractVisceral leishmaniasis is a major neglected tropical disease, with an estimated 500,000 new cases and more than 50,000 deaths attributable to this disease every year. Drug therapy is available but costly and resistance against several drug classes has evolved. Despite all efforts, no commercial, let alone affordable, vaccine is available to date. Thus, the development of cost effective, needle-independent vaccines is a high priority. Here, we have continued efforts to develop live vaccine carriers based on recombinant Salmonella. We used an in silico approach to select novel Leishmania parasite antigens from proteomic data sets, with selection criteria based on protein abundance, conservation across Leishmania species and low homology to host species. Five chosen antigens were differentially expressed on the surface or in the cytosol of Salmonella typhimurium SL3261. A two-step procedure was developed to select optimal Salmonella vaccine strains for each antigen, based on bacterial fitness and antigen expression levels. We show that vaccine strains of Salmonella expressing the novel Leishmania antigens LinJ08.1190 and LinJ23.0410 significantly reduced visceralisation of L. major and enhanced systemic resistance against L. donovani in susceptible BALB/c mice. The results show that Salmonella are valid vaccine carriers for inducing resistance against visceral leishmaniasis but that their use may not be suitable for all antigens.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectFemaleeng
dc.subjectMiceeng
dc.subjectGenetic Vectorseng
dc.subjectMice Inbred BALB Ceng
dc.subjectDisease Models Animaleng
dc.subjectLeishmania major/immunologyeng
dc.subjectAntigens Protozoan/geneticseng
dc.subjectAntigens Protozoan/immunologyeng
dc.subjectDrug Carriers/administration & dosageeng
dc.subjectLeishmania donovani/geneticseng
dc.subjectLeishmania donovani/immunologyeng
dc.subjectLeishmania major/geneticseng
dc.subjectLeishmaniasis Vaccines/administration & dosageeng
dc.subjectLeishmaniasis Vaccines/geneticseng
dc.subjectLeishmaniasis Vaccines/immunologyeng
dc.subjectLeishmaniasis Visceral/immunologyeng
dc.subjectLeishmaniasis Visceral/parasitologyeng
dc.subjectLeishmaniasis Visceral/prevention & controleng
dc.subjectSalmonella typhimurium/geneticseng
dc.subjectVaccines Synthetic/administration & dosageeng
dc.subjectVaccines Synthetic/geneticseng
dc.subjectVaccines Synthetic/immunologyeng
dc.subject.ddc610 Medizin
dc.titleSingle Dose Novel Salmonella Vaccine Enhances Resistance against Visceralizing L. major and L. donovani Infection in Susceptible BALB/c Mice.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10017349
dc.identifier.doi10.1371/journal.pntd.0001406
dc.identifier.doihttp://dx.doi.org/10.25646/966
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.container-textSchroeder J, Brown N, Kaye P, Aebischer T (2011) Single Dose Novel Salmonella Vaccine Enhances Resistance against Visceralizing L. major and L. donovani Infection in Susceptible BALB/c Mice. PLoS Negl Trop Dis 5(12): e1406.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001406
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume5
local.edoc.container-issue12
local.edoc.container-year2011

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