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2023-01-11Zeitschriftenartikel
Comparison of a Genotype 1 and a Genotype 2 Macaque Foamy Virus env Gene Indicates Distinct Infectivity and Cell-Cell Fusion but Similar Tropism and Restriction of Cell Entry by Interferon-Induced Transmembrane Proteins
dc.contributor.authorFricke, Thomas
dc.contributor.authorSchlagowski, Sarah
dc.contributor.authorLiu, Shanchuan
dc.contributor.authorYang, Xiaoling
dc.contributor.authorFiebig, Uwe
dc.contributor.authorKaul, Artur
dc.contributor.authorEnsser, Armin
dc.contributor.authorHahn, Alexander S.
dc.date.accessioned2023-11-28T09:34:45Z
dc.date.available2023-11-28T09:34:45Z
dc.date.issued2023-01-11none
dc.identifier.other10.3390/v15020262
dc.identifier.urihttp://edoc.rki.de/176904/11392
dc.description.abstractFoamy viruses (FVs) are naturally found in many different animals and also in primates with the notable exception of humans, but zoonotic infections are common. In several species, two different envelope (env) gene sequence clades or genotypes exist. We constructed a simian FV (SFV) clone containing a reporter gene cassette. In this background, we compared the env genes of the SFVmmu-DPZ9524 (genotype 1) and of the SFVmmu_R289hybAGM (genotype 2) isolates. SFVmmu_R289hybAGM env-driven infection was largely resistant to neutralization by SFVmmu-DPZ9524-neutralizing sera. While SFVmmu_R289hybAGM env consistently effected higher infectivity and cell-cell fusion, we found no differences in the cell tropism conferred by either env across a range of different cells. Infection by both viruses was weakly and non-significantly enhanced by simultaneous knockout of interferon-induced transmembrane proteins (IFITMs) 1, 2, and 3 in A549 cells, irrespective of prior interferon stimulation. Infection was modestly reduced by recombinant overexpression of IFITM3, suggesting that the SFV entry step might be weakly restricted by IFITM3 under some conditions. Overall, our results suggest that the different env gene clades in macaque foamy viruses induce genotype-specific neutralizing antibodies without exhibiting overt differences in cell tropism, but individual env genes may differ significantly with regard to fitnesseng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectfoamy viruseng
dc.subjectinterferon-induced transmembrane proteinseng
dc.subjectenvelopeeng
dc.subjectvirus entryeng
dc.subjectfusioneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleComparison of a Genotype 1 and a Genotype 2 Macaque Foamy Virus env Gene Indicates Distinct Infectivity and Cell-Cell Fusion but Similar Tropism and Restriction of Cell Entry by Interferon-Induced Transmembrane Proteinsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11392-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.container-issn1999-4915none
local.edoc.pages19none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-urlhttps://www.mdpi.com/journal/virusesnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume15none
local.edoc.container-issue2none
local.edoc.container-reportyear2023none
local.edoc.container-periodicalpart-titleSpecial Issue "Molecular Biology of RNA Viruses"none
dc.description.versionPeer Reviewednone

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