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2011-02-10Zeitschriftenartikel DOI: 10.1016/j.ijfoodmicro.2011.02.005
Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique
dc.contributor.authorHedderich, Reiner
dc.contributor.authorMüller, Rolf
dc.contributor.authorGreulich, Yasmin
dc.contributor.authorBannert, Norbert
dc.contributor.authorHolland, Gudrun
dc.contributor.authorKaiser, Petra
dc.contributor.authorReissbrodt, Rolf
dc.date.accessioned2018-05-07T15:28:17Z
dc.date.available2018-05-07T15:28:17Z
dc.date.created2012-03-19
dc.date.issued2011-02-10none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re0sXa3uYX2cE/PDF/22ESoOQNcEIn2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1158
dc.description.abstractColony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10 s, compared with short time hand plating (5 s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to control samples. The data clearly shows that the mechanical forces during hand plating are sufficient to damage E. coli cells.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectSurface-spread methodeng
dc.subjectSpiral platingeng
dc.subjectMechanical damage of bacterial cellseng
dc.subjectPlate counteng
dc.subjectBacteriological Techniques/methodseng
dc.subjectolony Count Microbialeng
dc.subjectGram-Negative Bacteria/growth & developmenteng
dc.subjectGram-Negative Bacteria/ultrastructureeng
dc.subjectMicroscopy Electroneng
dc.subject.ddc610 Medizin
dc.titleMechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10021925
dc.identifier.doi10.1016/j.ijfoodmicro.2011.02.005
dc.identifier.doihttp://dx.doi.org/10.25646/1083
local.edoc.container-titleInternational Journal of Food Microbiology
local.edoc.container-textHedderich, R., Müller, R., Greulich, Y., Bannert, N., Holland, G., Kaiser, P., Reissbrodt, R. Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique (2011) International Journal of Food Microbiology, 146 (1), pp. 105-107.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0168160511000729
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume146
local.edoc.container-issue1
local.edoc.container-year2011

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