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2011-04-28Zeitschriftenartikel DOI: 10.1016/j.jviromet.2011.04.025
Generation and characterisation of monoclonal antibodies against influenza virus A, subtype H5N1
dc.contributor.authorLinke, Sonja
dc.contributor.authorNeubauer, Katrin
dc.contributor.authorDorner, Martin
dc.contributor.authorDorner, Brigitte
dc.contributor.authorPauli, Georg
dc.contributor.authorSchweiger, Brunhilde
dc.date.accessioned2018-05-07T15:39:25Z
dc.date.available2018-05-07T15:39:25Z
dc.date.created2012-04-30
dc.date.issued2011-04-28none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rePmQ6jgfh7o/PDF/20yqxAd4DeYk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1219
dc.description.abstractThe emergence of highly pathogenic avian influenza A virus (HPAIV) subtype H5N1 in 1997 has since resulted in large outbreaks in poultry and in transmission from poultry to humans, mostly in southeast Asia, but also in several European countries. Effective diagnosis and control measures are essential for the management of HPAIV infections. To develop a rapid diagnostic test, a panel of murine monoclonal antibodies (mAbs) against influenza virus A subtype H5 was generated. Eleven mAbs were produced and characterised according to their reactivity by indirect and sandwich ELISA and western blotting against different H5 subtypes representing past and viruses currently circulating. Ten out of 11 mAbs reacted strongly with the haemagglutinin (HA) protein of H5 viruses, whereas one mAb reacted with the M1 protein. Targeted HA protein epitopes seemed to be conformational. One hybridoma clone binds to a linear epitope of the M1 protein. One specific mAb reacts with HPAIV H5 in the immunofluorescence test, and two antibodies neutralised H5 viruses. On the basis of the results, the set of seven mAbs is appropriate for developing diagnostic tests. With the generated mAbs, a sandwich ELISA was developed recognising all H5N1 strains tested but no other influenza viruses. With this ELISA, as little as 0.005 HA units or 0.1 ng/ml H5N1 was detected, surpassing other ELISA tests. The novel reagents have the potential to improve significantly available rapid antigen detection systems.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger; Robert Koch-Institut, Biologische Sicherheit
dc.subjectAvian influenza viruseng
dc.subjectH5N1eng
dc.subjectMonoclonal antibodyeng
dc.subjectCharacterisationeng
dc.subjectRapid antigen detectioneng
dc.subject.ddc610 Medizin
dc.titleGeneration and characterisation of monoclonal antibodies against influenza virus A, subtype H5N1
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10024316
dc.identifier.doi10.1016/j.jviromet.2011.04.025
dc.identifier.doihttp://dx.doi.org/10.25646/1144
local.edoc.container-titleJournal of Virological Methods
local.edoc.container-textLinke, S., Neubauer, K., Dorner, M.B., Dorner, B.G., Pauli, G., Schweiger, B. Generation and characterisation of monoclonal antibodies against influenza virus A, subtype H5N1 (2011) Journal of Virological Methods, 175 (1), pp. 85-94.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0166093411001637
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume175
local.edoc.container-issue1
local.edoc.container-year2011

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