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2012-06-26Zeitschriftenartikel DOI: 10.1371/journal.pntd.0001706
First International External Quality Assessment of Molecular Detection of Crimean-Congo Hemorrhagic Fever Virus
dc.contributor.authorEscadafal, Camille
dc.contributor.authorÖlschläger, Stephan
dc.contributor.authorAvšič-Županc, Tatjana
dc.contributor.authorPapa, Anna
dc.contributor.authorVanhomwegen, Jessica
dc.contributor.authorWölfel, Roman
dc.contributor.authorMirazimi, Ali
dc.contributor.authorTeichmann, Anette
dc.contributor.authorMantke, Oliver Donoso
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2018-05-07T15:44:54Z
dc.date.available2018-05-07T15:44:54Z
dc.date.created2012-06-28
dc.date.issued2012-06-26none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/retJIT8SPmY8Q/PDF/23YvQh0IxGrBg.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1249
dc.description.abstractCrimean-Congo hemorrhagic fever (CCHF) is a zoonosis caused by a Nairovirus of the family Bunyaviridae. Infection is transmitted to humans mostly by Hyalomma ticks and also by direct contact with the blood or tissues of infected humans or viremic livestock. Clinical features usually include a rapid progression characterized by hemorrhage, myalgia and fever, with a lethality rate up to 30%. CCHF is one of the most widely distributed viral hemorrhagic fevers and has been reported in Africa, the Middle East and Asia, as well as parts of Europe. There is no approved vaccine or specific treatment against CCHF virus (CCHFV) infections. In this context, an accurate diagnosis as well as a reliable surveillance of CCHFV infections is essential. Diagnostic techniques include virus culture, serology and molecular methods, which are now increasingly used. The European Network for the Diagnostics of “Imported” Viral Diseases organized the first international external quality assessment of CCHVF molecular diagnostics in 2011 to assess the efficiency and accurateness of CCHFV molecular methods applied by expert laboratories. A proficiency test panel of 15 samples was distributed to the participants including 10 different CCHFV preparations generated from infected cell cultures, a preparation of plasmid cloned with the nucleoprotein of CCHFV, two CCHFV RNA preparations and two negative controls. Forty-four laboratories worldwide participated in the EQA study and 53 data sets were received. Twenty data sets (38%) met all criteria with optimal performance, 10 (19%) with acceptable performance, while 23 (43%) reported results showing a need for improvement. Differences in performance depended on the method used, the type of strain tested, the concentration of the sample tested and the laboratory performing the test. These results indicate that there is still a need for improving testing conditions and standardizing protocols for the molecular detection of Crimean-Congo hemorrhagic fever virus.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectVirology/methodseng
dc.subjectInternational Cooperationeng
dc.subjectClinical Laboratory Techniques/standardseng
dc.subjectMolecular Diagnostic Techniques/methodseng
dc.subjectVirology/standardseng
dc.subjectClinical Laboratory Techniques/methodseng
dc.subjectHemorrhagic Fever Crimean/diagnosiseng
dc.subjectLaboratory Proficiency Testingeng
dc.subjectMolecular Diagnostic Techniques/standardseng
dc.subjectNairovirus/isolation & purificationeng
dc.subjectQuality Assurance Health Careeng
dc.subject.ddc610 Medizin
dc.titleFirst International External Quality Assessment of Molecular Detection of Crimean-Congo Hemorrhagic Fever Virus
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10025801
dc.identifier.doi10.1371/journal.pntd.0001706
dc.identifier.doihttp://dx.doi.org/10.25646/1174
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.container-textEscadafal C, Ölschläger S, Avšič-Županc T, Papa A, Vanhomwegen J, et al. (2012) First International External Quality Assessment of Molecular Detection of Crimean-Congo Hemorrhagic Fever Virus. PLoS Negl Trop Dis 6(6): e1706.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001706
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume6
local.edoc.container-issue6
local.edoc.container-year2012

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