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2012-06-13Zeitschriftenartikel DOI: 10.1371/journal.pone.0038709
Mycobacterium tuberculosis DosR Regulon Gene Rv0079 Encodes a Putative, ‘Dormancy Associated Translation Inhibitor (DATIN)’
dc.contributor.authorKumar, Ashutosh
dc.contributor.authorMajid, Mohammad
dc.contributor.authorKunisch, Ralph
dc.contributor.authorRani, Pittu Sandhya
dc.contributor.authorQureshi, Insaf A.
dc.contributor.authorLewin, Astrid
dc.contributor.authorHasnain, Seyed E.
dc.contributor.authorAhmed, Niyaz
dc.date.accessioned2018-05-07T15:47:06Z
dc.date.available2018-05-07T15:47:06Z
dc.date.created2012-08-22
dc.date.issued2012-06-13none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reTWqJ3dUbsSo/PDF/20VE7dLv6Jdec.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1261
dc.description.abstractMycobacterium tuberculosis is a major human pathogen that has evolved survival mechanisms to persist in an immune-competent host under a dormant condition. The regulation of M. tuberculosis metabolism during latent infection is not clearly known. The dormancy survival regulon (DosR regulon) is chiefly responsible for encoding dormancy related functions of M. tuberculosis. We describe functional characterization of an important gene of DosR regulon, Rv0079, which appears to be involved in the regulation of translation through the interaction of its product with bacterial ribosomal subunits. The protein encoded by Rv0079, possibly, has an inhibitory role with respect to protein synthesis, as revealed by our experiments. We performed computational modelling and docking simulation studies involving the protein encoded by Rv0079 followed by in vitro translation and growth curve analysis experiments, involving recombinant E. coli and Bacille Calmette Guérin (BCG) strains that overexpressed Rv0079. Our observations concerning the interaction of the protein with the ribosomes are supportive of its role in regulation/inhibition of translation. We propose that the protein encoded by locus Rv0079 is a ‘dormancy associated translation inhibitor’ or DATIN.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectBacterial Proteins/geneticseng
dc.subjectDNA Primerseng
dc.subjectBase Sequenceeng
dc.subjectMycobacterium tuberculosis/geneticseng
dc.subjectBlotting Westerneng
dc.subjectModels Moleculareng
dc.subjectGenes Bacterialeng
dc.subjectProtein Kinases/geneticseng
dc.subjectReguloneng
dc.subject.ddc610 Medizin
dc.titleMycobacterium tuberculosis DosR Regulon Gene Rv0079 Encodes a Putative, ‘Dormancy Associated Translation Inhibitor (DATIN)’
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026653
dc.identifier.doi10.1371/journal.pone.0038709
dc.identifier.doihttp://dx.doi.org/10.25646/1186
local.edoc.container-titlePLoS ONE
local.edoc.container-textKumar A, Majid M, Kunisch R, Rani PS, Qureshi IA, et al. (2012) Mycobacterium tuberculosis DosR Regulon Gene Rv0079 Encodes a Putative, ‘Dormancy Associated Translation Inhibitor (DATIN)’. PLoS ONE 7(6): e38709.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038709
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue6
local.edoc.container-year2012

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