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2012-01-01Zeitschriftenartikel DOI: 10.4161/pri.6.1.18556
Harnessing prions as test agents for the development of broad-range disinfectants
dc.contributor.authorWagenführ, Katja
dc.contributor.authorBeekes, Michael
dc.date.accessioned2018-05-07T15:49:19Z
dc.date.available2018-05-07T15:49:19Z
dc.date.created2012-09-04
dc.date.issued2012-01-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rec9EeVpphGBY/PDF/27pHUHrM6wUM.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1273
dc.description.abstractThe development of disinfectants with broad-range efficacy against bacteria, viruses, fungi, protozoa and prions constitutes an ongoing challenge. Prions, the causative agents of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease (CJD) or its variant (vCJD) rank among the pathogens with the highest resistance to disinfection. Pilot studies have shown that different procedures devised for prion disinfection were also highly effective against microbial pathogens. This fueled the idea to systematically exploit prions as test pathogens for the identification of new potential broad-range disinfectants. Prions essentially consist of misfolded, aggregated prion protein (PrP) and putatively replicate by nucleation-dependent, or seeded PrP polymerization. Recently, we have been able to establish PrP seeding activity as a quantitative in vitro indicator for the disinfection of 263K scrapie prions on steel wires used as surrogates for medical instruments. The seeding activity on wires re-processed in different disinfectants could be (1) biochemically determined by quantitative protein misfolding cyclic amplification (qPMCA), (2) biologically detected after qPMCA in a cell assay and (3) correctly translated into residual titers of scrapie infectivity. Our approach will substantially facilitate the identification of disinfectants with efficacy against prions as promising candidates for a further microbiological validation of broad-range activity.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectDisinfectants/pharmacologyeng
dc.subjectDisinfection/methodseng
dc.subjectFungi/drug effectseng
dc.subjectBacteria/drug effectseng
dc.subjectBacteria/isolation & purificationeng
dc.subjectFungi/isolation & purificationeng
dc.subjectPrions/pharmacologyeng
dc.subjectSurgical Instrumentseng
dc.subject.ddc610 Medizin
dc.titleHarnessing prions as test agents for the development of broad-range disinfectants
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026791
dc.identifier.doi10.4161/pri.6.1.18556
dc.identifier.doihttp://dx.doi.org/10.25646/1198
local.edoc.container-titlePrion
local.edoc.container-textWagenführ, K., Beekes, M. Harnessing prions as test agents for the development of broad-range disinfectants (2012) Prion, 6 (1), pp. 1-6.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.landesbioscience.com/journals/prion/article/18556/?nocache=1062234713
local.edoc.container-publisher-nameLandes Bioscience
local.edoc.container-volume6
local.edoc.container-issue1
local.edoc.container-year2012

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