Show simple item record

2011-08-27Zeitschriftenartikel DOI: 10.1016/j.ijfoodmicro.2011.08.020
Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany
dc.contributor.authorHauser, Elisabeth
dc.contributor.authorHebner, Franka
dc.contributor.authorTietze, Erhard
dc.contributor.authorHelmuth, Reiner
dc.contributor.authorJunker, Ernst
dc.contributor.authorPrager, Rita
dc.contributor.authorSchroeter, Andreas
dc.contributor.authorRabsch, Wolfgang
dc.contributor.authorFruth, Angelika
dc.contributor.authorMalorny, Burkhard
dc.date.accessioned2018-05-07T15:50:37Z
dc.date.available2018-05-07T15:50:37Z
dc.date.created2012-09-07
dc.date.issued2011-08-27none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/relwUuZVVgNA/PDF/22lZucboIw4vk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1280
dc.description.abstractSalmonella enterica serovar Derby (S. Derby) is one of the most prevalent serovars in pigs in Europe and in the U.S. and ranks among the 10 most frequently isolated serovars in humans. Therefore, a set of 82 epidemiologically unrelated S. Derby strains isolated between 2006 and 2008 from pigs, pork and humans in Germany was selected and investigated in respect to the transmission of clonal groups of the serovar along the food chain. Various phenotypic and genotypic methods were applied and the pathogenicity and resistance gene repertoire was determined. Phenotypically 72% of the strains were susceptible to all 17 antimicrobials tested while the others were monoresistant to tetracycline or multi-resistant with different resistance profiles. Four major clonal groups were identified based on PFGE, sequence data of the virulence genes sopA, sopB and sopD, VNTR-locus STTR5 and MLST revealing also the new sequence type ST774. Thirty different PFGE profiles were detected resulting in four clusters representing the four groups. The pathogenicity gene repertoire of 32 representative S. Derby strains analyzed by microarray showed six types with differences in the Salmonella pathogenicity islands, pathogenicity genes on smaller islets or prophages and fimbriae coding genes. The pathogenicity gene repertoire of the predominant types PAT DE1 and DE2 were most similar to the ones of S. Paratyphi B (dT+, O5−) and to a minor degree to S. Infantis and S. Virchow PATs. Overall this study showed that in Germany currently one major S. Derby clone is frequently isolated from pigs and humans. Contaminated pork was identified as one vehicle and consequently is a risk for human health. To prevent this serovar from entering the food chain, control measurements should be applied at the farm level.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectSalmonellaeng
dc.subjectSerovar S. Derbyeng
dc.subjectPulsed-field gel electrophoresiseng
dc.subjectMicroarray analysiseng
dc.subjectMultilocus sequence typingeng
dc.subjectAntimicrobial resistanceeng
dc.subject.ddc610 Medizin
dc.titleDiversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026877
dc.identifier.doi10.1016/j.ijfoodmicro.2011.08.020
dc.identifier.doihttp://dx.doi.org/10.25646/1205
local.edoc.container-titleInternational Journal of Food Microbiology
local.edoc.container-textHauser, E., Hebner, F., Tietze, E., Helmuth, R., Junker, E., Prager, R., Schroeter, A., Rabsch, W., Fruth, A., Malorny, B. Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany (2011) International Journal of Food Microbiology, 151 (2), pp. 141-149.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0168160511004934
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume151
local.edoc.container-issue2
local.edoc.container-year2011

Show simple item record