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2011-05-30Zeitschriftenartikel DOI: 10.1016/j.jviromet.2011.04.028
Application of a split luciferase complementation assay for the detection of viral protein–protein interactions
dc.contributor.authorDeng, Qiji
dc.contributor.authorWang, Dan
dc.contributor.authorXiang, Xiaoxiao
dc.contributor.authorGao, Xiaofei
dc.contributor.authorHardwidge, Philip R.
dc.contributor.authorKaushik, Radhey S.
dc.contributor.authorWolff, Thorsten
dc.contributor.authorChakravarty, Suvobrata
dc.contributor.authorLi, Feng
dc.date.accessioned2018-05-07T15:53:22Z
dc.date.available2018-05-07T15:53:22Z
dc.date.created2012-09-14
dc.date.issued2011-05-30none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re52c9PTnMVZw/PDF/29H4qgTpM6lK2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1295
dc.description.abstractIntraviral protein-protein interactions are critical for virus survival in the host. Discovery of such interactions is important to understand molecular mechanisms of viral replication and pathogenesis. The development of a cell-based assay that can be employed to examine systematically viral protein interactions is described. The method, known as the split luciferase complementation assay (SLCA), is based on the principle that N- and C-terminal domains of luciferase alone do not emit luminescence; however, if fused to interacting proteins the two non-functional halves can be brought into close enough proximity through a specific protein-protein interaction to restore the functions of the enzyme and emit detectable light. The well-studied influenza B polymerase acidic protein (PA) and basic protein 1 (PB1) interaction was used as a model system to develop the assay. Consistent with previous studies, a strong PA-PB1 interaction was demonstrated in the assay. The PA-PB1 interaction was also disrupted by single amino acid mutations in the N-terminal domain of PB1 that is responsible for binding PA. The described SLCA is highly specific and easy to perform, and thus may be useful for studying protein-protein interactions in viral diseases.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectViral diseaseeng
dc.subjectProtein–protein interactioneng
dc.subjectSplit luciferase complementation assayeng
dc.subject.ddc610 Medizin
dc.titleApplication of a split luciferase complementation assay for the detection of viral protein–protein interactions
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027088
dc.identifier.doi10.1016/j.jviromet.2011.04.028
dc.identifier.doihttp://dx.doi.org/10.25646/1220
local.edoc.container-titleJournal of Virological Methods
local.edoc.container-textQiji Deng, Dan Wang, Xiaoxiao Xiang, Xiaofei Gao, Philip R. Hardwidge, Radhey S. Kaushik, Thorsten Wolff, Suvobrata Chakravarty, Feng Li Application of a split luciferase complementation assay for the detection of viral protein–protein interactions (2011) Journal of Virological Methods, 176 (1-2), pp. 108-111.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0166093411001662
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume176
local.edoc.container-issue1-2
local.edoc.container-year2011

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