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2009-11-18Zeitschriftenartikel DOI: 10.1099/vir.0.016022-0
Deletion of the rat cytomegalovirus immediate-early 1 gene results in a virus capable of establishing latency, but with lower levels of acute virus replication and latency that compromise reactivation efficiency
dc.contributor.authorSandford, Gordon R.
dc.contributor.authorSchumacher, Uwe
dc.contributor.authorEttinger, Jakob
dc.contributor.authorBrune, Wolfram
dc.contributor.authorHayward, Gary S.
dc.contributor.authorBurns, William H.
dc.contributor.authorVoigt, Sebastian
dc.date.accessioned2018-05-07T15:57:23Z
dc.date.available2018-05-07T15:57:23Z
dc.date.created2012-10-02
dc.date.issued2009-11-18none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re8gfILLgXQ/PDF/21k1uuR70ErZw.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1317
dc.description.abstractThe immediate-early 1 (IE1) and IE2 proteins encoded by the major immediate-early (MIE) transcription unit of cytomegaloviruses are thought to play key roles in the switch between latent- and lytic-cycle infection. Whilst IE2 is essential for triggering the lytic cycle, the exact roles of IE1 have not been resolved. An MIE-exon 4-deleted rat cytomegalovirus (DeltaIE1) failed to synthesize the IE1 protein and did not disperse promyelocytic leukaemia bodies early post-infection, but was still capable of normal replication in fibroblast cell culture. However, DeltaIE1 had a diminished ability to infect salivary glands persistently in vivo and to reactivate from spleen explant cultures ex vivo. Quantification of viral genomes in spleens of infected animals revealed a reduced amount of DeltaIE1 virus produced during acute infection, suggesting a role for IE1 as a regulator in establishing a chronic or persistent infection, rather than in influencing the latency or reactivation processes more directly.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectVirulenceeng
dc.subjectCellseng
dc.subjectCulturedeng
dc.subjectVirus Replicationeng
dc.subjectMuromegalovirus/geneticseng
dc.subjectRatseng
dc.subjectVirus Activationeng
dc.subjectFibroblasts/virologyeng
dc.subjectHerpesviridae Infections/virologyeng
dc.subjectImmediate-Early Proteins/geneticseng
dc.subjectMuromegalovirus/physiologyeng
dc.subjectSalivary Glands/virologyeng
dc.subjectSequence Deletioneng
dc.subjectSpleen/virologyeng
dc.subjectTrans-Activators/geneticseng
dc.subjectVirus Latencyeng
dc.subjectDNA Viral/geneticseng
dc.subject.ddc610 Medizin
dc.titleDeletion of the rat cytomegalovirus immediate-early 1 gene results in a virus capable of establishing latency, but with lower levels of acute virus replication and latency that compromise reactivation efficiency
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027352
dc.identifier.doi10.1099/vir.0.016022-0
dc.identifier.doihttp://dx.doi.org/10.25646/1242
local.edoc.container-titleJournal of General Virology
local.edoc.container-textGordon R. Sandford, Uwe Schumacher, Jakob Ettinger, Wolfram Brune, Gary S. Hayward, William H. Burns and Sebastian Voigt. Deletion of the rat cytomegalovirus immediate-early 1 gene results in a virus capable of establishing latency, but with lower levels of acute virus replication and latency that compromise reactivation efficiency. (2010) Journal of General Virology, 91, pp. 616–621.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://vir.sgmjournals.org/content/91/3/616
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume91
local.edoc.container-issue3
local.edoc.container-year2010

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