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2009-02-25Zeitschriftenartikel DOI: 10.1074/jbc.M900773200
Cross-talk between type three secretion system and metabolism in Yersinia.
dc.contributor.authorSchmid, Annika
dc.contributor.authorNeumayer, Wibke
dc.contributor.authorTrülzsch, Konrad
dc.contributor.authorIsrael, Lars
dc.contributor.authorImhof, Axel
dc.contributor.authorRoessle, Manfred
dc.contributor.authorSauer, Guido
dc.contributor.authorRichter, Susanna
dc.contributor.authorLauw, Susan
dc.contributor.authorEylert, Eva
dc.contributor.authorEisenreich, Wolfgang
dc.contributor.authorHeesemann, Jürgen
dc.contributor.authorWilharm, Gottfried
dc.date.accessioned2018-05-07T16:02:34Z
dc.date.available2018-05-07T16:02:34Z
dc.date.created2012-11-05
dc.date.issued2009-02-25none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reMZoJ3jO9FxI/PDF/206O9yAD21mo.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1345
dc.description.abstractPathogenic yersiniae utilize a type three secretion system (T3SS) to inject Yop proteins into host cells in order to undermine their immune response. YscM1 and YscM2 proteins have been reported to be functionally equivalent regulators of the T3SS in Yersinia enterocolitica. Here, we show by affinity purification, native gel electrophoresis and small angle x-ray scattering that both YscM1 and YscM2 bind to phosphoenolpyruvate carboxylase (PEPC) of Y. enterocolitica. Under in vitro conditions, YscM1, but not YscM2, was found to inhibit PEPC with an apparent IC(50) of 4 mum (K(i) = 1 mum). To analyze the functional roles of PEPC, YscM1, and YscM2 in Yop-producing bacteria, cultures of Y. enterocolitica wild type and mutants defective in the formation of PEPC, YscM1, or YscM2, respectively, were grown under low calcium conditions in the presence of [U-(13)C(6)]glucose. The isotope compositions of secreted Yop proteins and nine amino acids from cellular proteins were analyzed by mass spectrometry. The data indicate that a considerable fraction of oxaloacetate used as precursor for amino acids was derived from [(13)C(3)]phosphoenolpyruvate by the catalytic action of PEPC in the wild-type strain but not in the PEPC(-) mutant. The data imply that PEPC is critically involved in replenishing the oxaloacetate pool in the citrate cycle under virulence conditions. In the YscM1(-) and YscM2(-) mutants, increased rates of pyruvate formation via glycolysis or the Entner-Doudoroff pathway, of oxaloacetate formation via the citrate cycle, and of amino acid biosynthesis suggest that both regulators trigger the central metabolism of Y. enterocolitica. We propose a "load-and-shoot cycle" model to account for the cross-talk between T3SS and metabolism in pathogenic yersiniae.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectBacterial Proteins/geneticseng
dc.subjectBacterial Proteins/metabolismeng
dc.subjectYersinia enterocolitica/geneticseng
dc.subjectYersinia enterocolitica/metabolismeng
dc.subjectTranscription Factors/geneticseng
dc.subjectTranscription Factors/metabolismeng
dc.subjectYersinia enterocolitica/pathogenicityeng
dc.subjectGlucose/metabolismeng
dc.subjectAmino Acids/biosynthesiseng
dc.subjectAmino Acids/geneticseng
dc.subjectCalcium/metabolismeng
dc.subjectCalcium/pharmacologyeng
dc.subjectCitric Acid Cycle/drug effectseng
dc.subjectCitric Acid Cycle/physiologyeng
dc.subjectGlucose/pharmacologyeng
dc.subjectGlycolysis/drug effectseng
dc.subjectGlycolysis/physiologyeng
dc.subjectOxaloacetate/metabolismeng
dc.subjectPhosphoenolpyruvate Carboxylase/geneticseng
dc.subjectPhosphoenolpyruvate Carboxylase/metabolismeng
dc.subjectPyruvic Acid/metabolismeng
dc.subjectSecretory Pathway/drug effectseng
dc.subjectSecretory Pathway/physiologyeng
dc.subjectSweetening Agents/metabolismeng
dc.subjectSweetening Agents/pharmacologyeng
dc.subject.ddc610 Medizin
dc.titleCross-talk between type three secretion system and metabolism in Yersinia.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027777
dc.identifier.doi10.1074/jbc.M900773200
dc.identifier.doihttp://dx.doi.org/10.25646/1270
local.edoc.container-titleThe Journal of Biological Chemistry
local.edoc.container-textAnnika Schmid, Wibke Neumayer, Konrad Trülzsch, Lars Israel, Axel Imhof, Manfred Roessle, Guido Sauer, Susanna Richter, Susan Lauw, Eva Eylert, Wolfgang Eisenreich, Jürgen Heesemann and Gottfried Wilharm. Cross-talk between Type Three Secretion System and Metabolism in Yersinia. (2009) The Journal of Biological Chemistry , 284, pp. 12165-12177.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.jbc.org/content/284/18/12165
local.edoc.container-publisher-nameAmerican Society for Biochemistry and Molecular Biology
local.edoc.container-volume284
local.edoc.container-issue18
local.edoc.container-year2009

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