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2012-05-14Zeitschriftenartikel DOI: 10.1155/2012/156035
Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry
dc.contributor.authorFörster, Charlotte
dc.contributor.authorEichert, André
dc.contributor.authorOberthür, Dominik
dc.contributor.authorBetzel, Christian
dc.contributor.authorGeßner, Reinhard
dc.contributor.authorNitsche, Andreas
dc.contributor.authorFürste, Jens P.
dc.date.accessioned2018-05-07T16:05:13Z
dc.date.available2018-05-07T16:05:13Z
dc.date.created2012-11-13
dc.date.issued2012-05-14none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reC4MOypBmmk/PDF/22XG9RO0GcR2I.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1359
dc.description.abstract"Locked nucleic acids" (LNAs) belong to the backbone-modified nucleic acid family. The 2'-O,4'-C-methylene-β-D-ribofuranose nucleotides are used for single or multiple substitutions in RNA molecules and thereby introduce enhanced bio- and thermostability. This renders LNAs powerful tools for diagnostic and therapeutic applications. RNA molecules maintain the overall canonical A-type conformation upon substitution of single or multiple residues/nucleotides by LNA monomers. The structures of "all" LNA homoduplexes, however, exhibit significant differences in their overall geometry, in particular a decreased twist, roll and propeller twist. This results in a widening of the major groove, a decrease in helical winding, and an enlarged helical pitch. Therefore, the LNA duplex structure can no longer be described as a canonical A-type RNA geometry but can rather be brought into proximity to other backbone-modified nucleic acids, like glycol nucleic acids or peptide nucleic acids. LNA-modified nucleic acids provide thus structural and functional features that may be successfully exploited for future application in biotechnology and drug discovery.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subject.ddc610 Medizin
dc.titleFeatures of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10028432
dc.identifier.doi10.1155/2012/156035
dc.identifier.doihttp://dx.doi.org/10.25646/1284
local.edoc.container-titleJournal of Nucleic Acids
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.hindawi.com/journals/jna/2012/156035/
local.edoc.container-publisher-nameHindawi Publishing Corporation
local.edoc.container-year2012

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