2012-12-06Zeitschriftenartikel DOI: 10.25646/1313
Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
Corman, V. M.
Müller, Marcel A.
Drexler, Jan Felix
Osterhaus, A. D.
Haagmans, B. L.
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.
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