Show simple item record

2013-01-01Zeitschriftenartikel DOI: 10.1007/978-3-642-33570-9_11
Complexity of Botulinum Neurotoxins: Challenges for Detection Technology
dc.contributor.authorDorner, Martin
dc.contributor.authorSchulz, Melanie
dc.contributor.authorKull, Skadi
dc.contributor.authorDorner, Brigitte
dc.date.accessioned2018-05-07T16:13:11Z
dc.date.available2018-05-07T16:13:11Z
dc.date.created2013-01-10
dc.date.issued2013-01-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reeYx7fJLjaS2/PDF/21sRreA6rqWu6.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1402
dc.description.abstractThe detection of botulinum neurotoxins (BoNT) is extremely challenging due to their high toxicity and the multiple BoNT variants. To date, seven serotypes with more than 30 subtypes have been described, and even more subtypes are expected to be discovered. The fact that the BoNT molecules are released as large complexes of different size and composition adds further complexity to the issue. Currently, in the diagnostics of botulism, the mouse bioassay (MBA) is still considered as gold standard for the detection of BoNT in complex sample materials. Over the years, different functional, immunological, and spectrometric assays or combinations thereof have been developed, supplemented by DNA-based assays for the detection of the organism. In this review, advantages and limitations of the current technologies will be discussed, highlighting some of the intricacies of real sample analysis.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectAnimalseng
dc.subjectMass Spectrometryeng
dc.subjectDNA Bacterial/analysiseng
dc.subjectDNA Bacterial/chemistryeng
dc.subjectBiosensing Techniqueseng
dc.subjectBotulinum Toxins/chemistryeng
dc.subjectBotulinum Toxins/geneticseng
dc.subjectBotulinum Toxins/isolation & purificationeng
dc.subjectBotulism/diagnosiseng
dc.subjectBotulism/microbiologyeng
dc.subjectClostridium botulinum/chemistryeng
dc.subjectClostridium botulinum/enzymologyeng
dc.subjectClostridium botulinum/geneticseng
dc.subjectClostridium botulinum/pathogenicityeng
dc.subjectEndopeptidases/chemistryeng
dc.subjectEnzyme Activationeng
dc.subjectEnzyme Assays/methodseng
dc.subjectImmunoassay/methodseng
dc.subjectMiceeng
dc.subjectNeurotoxins/chemistryeng
dc.subjectNeurotoxins/geneticseng
dc.subjectTime Factorseng
dc.subject.ddc610 Medizin
dc.titleComplexity of Botulinum Neurotoxins: Challenges for Detection Technology
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029182
dc.identifier.doi10.1007/978-3-642-33570-9_11
dc.identifier.doihttp://dx.doi.org/10.25646/1327
local.edoc.container-titleCurrent Topics in Microbiology and Immunology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameSpringer
local.edoc.container-issue364
local.edoc.container-year2013

Show simple item record