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2012-11-05Zeitschriftenartikel DOI: 10.1186/1743-422X-9-260
Increased titers of neutralizing antibodies after immunization with both envelope proteins of the porcine endogenous retroviruses (PERVs)
dc.contributor.authorDenner, Joachim
dc.contributor.authorMihica, Debora
dc.contributor.authorKaulitz, Danny
dc.contributor.authorSchmidt, Christa-Maria
dc.date.accessioned2018-05-07T16:15:01Z
dc.date.available2018-05-07T16:15:01Z
dc.date.created2013-03-01
dc.date.issued2012-11-05none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reqgVgG0ryyjM/PDF/23cndujFOvIYQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1412
dc.description.abstractDespite enormous difficulties to induce antibodies neutralizing HIV-1, especially broadly neutralizing antibodies directed against the conserved membrane proximal external region (MPER) of the transmembrane envelope protein, such antibodies can be easily induced in the case of gammaretroviruses, among them the porcine endogenous retroviruses (PERVs). In addition to neutralizing antibodies directed against the transmembrane envelope protein p15E, neutralizing antibodies were also induced by immunization with the surface envelope protein gp70. PERVs represent a special risk for xenotransplantation using pig tissues or organs since they are integrated in the genome of all pigs and infect human cells and a vaccine may protect from transmission to the recipient. To investigate the effect of simultaneous immunization with both proteins in detail, a study was performed in hamsters. Gp70 and p15E of PERV were produced in E. coli, purified and used for immunization. All animals developed binding antibodies against the antigens used for immunization. Sera from animals immunized with p15E recognized epitopes in the MPER and the fusion peptide proximal region (FPPR) of p15E. One MPER epitope showed a sequence homology to an epitope in the MPER of gp41 of HIV-1 recognized by broadly neutralizing antibodies found in HIV infected individuals. Neutralizing antibodies were detected in all sera. Most importantly, sera from animals immunized with gp70 had a higher neutralizing activity when compared with the sera from animals immunized with p15E and sera from animals immunized with gp70 together with p15E had a higher neutralizing activity compared with sera from animals immunized with each antigen alone. These immunization studies are important for the development of vaccines against other retroviruses including the human immunodeficiency virus HIV-1.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectNeutralizing antibodieseng
dc.subjectVaccineeng
dc.subjectHIV-1eng
dc.subjectPorcine Endogenous Retroviruses (PERV)eng
dc.subject.ddc610 Medizin
dc.titleIncreased titers of neutralizing antibodies after immunization with both envelope proteins of the porcine endogenous retroviruses (PERVs)
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029473
dc.identifier.doi10.1186/1743-422X-9-260
dc.identifier.doihttp://dx.doi.org/10.25646/1337
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/9/1/260
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume9
local.edoc.container-issue260
local.edoc.container-year2012

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