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2012-05-03Zeitschriftenartikel DOI: 10.1016/j.ijmm.2012.03.003
Surface-associated motility, a common trait of clinical isolates of Acinetobacter baumannii, depends on 1,3-diaminopropane
dc.contributor.authorSkiebe, Evelyn
dc.contributor.authorBerardinis, Véronique de
dc.contributor.authorMorczinek, Peter
dc.contributor.authorKerrinnes, Tobias
dc.contributor.authorFaber, Franziska
dc.contributor.authorLepka, Daniela
dc.contributor.authorHammer, Bettina
dc.contributor.authorZimmermann, Ortrud
dc.contributor.authorZiesing, Stefan
dc.contributor.authorWichelhaus, Thomas A.
dc.contributor.authorHunfeld, Klaus-Peter
dc.contributor.authorBorgmann, Stefan
dc.contributor.authorGröbner, Sabine
dc.contributor.authorHiggins, Paul G.
dc.contributor.authorSeifert, Harald
dc.contributor.authorBusse, Hans-Jürgen
dc.contributor.authorWitte, Wolfgang
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorWilharm, Gottfried
dc.date.accessioned2018-05-07T16:24:21Z
dc.date.available2018-05-07T16:24:21Z
dc.date.created2013-05-02
dc.date.issued2012-05-03none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reJgULrg0ffg/PDF/21reXarKdpzpc.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1462
dc.description.abstractWhile flagella-independent motility has long been described in representatives of the genus Acinetobacter, the mechanism of motility remains ambiguous. Acinetobacter baumannii, a nosocomial pathogen appearing increasingly multidrug-resistant, may profit from motility during infection or while persisting in the hospital environment. However, data on the frequency of motility skills among clinical A. baumannii isolates is scarce. We have screened a collection of 83 clinical A. baumannii isolates of different origin and found that, with the exception of one isolate, all were motile on wet surfaces albeit to varying degrees and exhibiting differing morphologies. Screening a collection of transposon mutants of strain ATCC 17978 for motility defects, we identified 2 akinetic mutants carrying transposon insertions in the dat and ddc gene, respectively. These neighbouring genes contribute to synthesis of 1,3-diaminopropane (DAP), a polyamine ubiquitously produced in Acinetobacter. Supplementing semi-solid media with DAP cured the motility defect of both mutants. HPLC analyses confirmed that DAP synthesis was abolished in ddc and dat mutants of different A. baumannii isolates and was re-established after genetic complementation. Both, the dat and ddc mutant of ATCC 17978 were attenuated in the Galleria mellonella caterpillar infection model. Taken together, surface-associated motility is a common trait of clinical A. baumannii isolates that requires DAP and may play a role in its virulence.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAcinetobacter baumanniieng
dc.subjectMotilityeng
dc.subject1eng
dc.subject3-Diaminopropaneeng
dc.subjectdateng
dc.subjectddceng
dc.subjectAcinetobacter baylyieng
dc.subjectADP1eng
dc.subjectGalleria mellonellaeng
dc.subject.ddc610 Medizin
dc.titleSurface-associated motility, a common trait of clinical isolates of Acinetobacter baumannii, depends on 1,3-diaminopropane
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10030253
dc.identifier.doi10.1016/j.ijmm.2012.03.003
dc.identifier.doihttp://dx.doi.org/10.25646/1387
local.edoc.container-titleInternational Journal of Medical Microbiology
local.edoc.container-textSkiebe, E., de Berardinis, V., Morczinek, P., Kerrinnes, T., Faber, F., Lepka, D., Hammer, B., Zimmermann, O., Ziesing, S., Wichelhaus, T.A., Hunfeld, K.-P., Borgmann, S., Gröbner, S., Higgins, P.G., Seifert, H., Busse, H.-J., Witte, W., Pfeifer, Y., Wilharm, G. Surface-associated motility, a common trait of clinical isolates of Acinetobacter baumannii, depends on 1,3-diaminopropane (2012) International Journal of Medical Microbiology, 302 (3), pp. 117-128.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S1438422112000161
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume302
local.edoc.container-issue3
local.edoc.container-year2012

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