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2013-09-06Zeitschriftenartikel DOI: 10.1371/journal.pone.0073927
Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic
dc.contributor.authorMarejková, Monika
dc.contributor.authorBláhová, Květa
dc.contributor.authorJanda, Jan
dc.contributor.authorFruth, Angelika
dc.contributor.authorPetráš, Petr
dc.date.accessioned2018-05-07T17:02:03Z
dc.date.available2018-05-07T17:02:03Z
dc.date.created2013-09-19
dc.date.issued2013-09-06none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reHaPzK4NFKQ/PDF/21zrRoFX4T36.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1668
dc.description.abstractBackground: Enterohemorrhagic Escherichia coli (EHEC) cause diarrhea-associated hemolytic uremic syndrome (D+ HUS) worldwide, but no systematic study of EHEC as the causative agents of HUS was performed in the Czech Republic. We analyzed stools of all patients with D+ HUS in the Czech Republic between 1998 and 2012 for evidence of EHEC infection. We determined virulence profiles, phenotypes, antimicrobial susceptibilities and phylogeny of the EHEC isolates. Methodology/Principal Findings: Virulence loci were identified using PCR, phenotypes and antimicrobial susceptibilities were determined using standard procedures, and phylogeny was assessed using multilocus sequence typing. During the 15-year period, EHEC were isolated from stools of 39 (69.4%) of 56 patients. The strains belonged to serotypes [fliC types] O157:H7/NM[fliCH7] (50% of which were sorbitol-fermenting; SF), O26:H11/NM[fliCH11], O55:NM[fliCH7], O111:NM[fliCH8], O145:H28[fliCH28], O172:NM[fliCH25], and Orough:NM[fliCH25]. O26:H11/NM[fliCH11] was the most common serotype associated with HUS (41% isolates). Five stx genotypes were identified, the most frequent being stx2a (71.1% isolates). Most strains contained EHEC-hlyA encoding EHEC hemolysin, and a subset (all SF O157:NM and one O157:H7) harbored cdt-V encoding cytolethal distending toxin. espPα encoding serine protease EspPα was found in EHEC O157:H7, O26:H11/NM, and O145:H28, whereas O172:NM and Orough:NM strains contained espPγ. All isolates contained eae encoding adhesin intimin, which belonged to subtypes β (O26), γ (O55, O145, O157), γ2/θ (O111), and ε (O172, Orough). Loci encoding other adhesins (efa1, lpfAO26, lpfAO157OI-141, lpfAO157OI-154, iha) were usually associated with particular serotypes. Phylogenetic analysis demonstrated nine sequence types (STs) which correlated with serotypes. Of these, two STs (ST660 and ST1595) were not found in HUS-associated EHEC before. Conclusions/Significance: EHEC strains, including O157:H7 and non-O157:H7, are frequent causes of D+ HUS in the Czech Republic. Identification of unusual EHEC serotypes/STs causing HUS calls for establishment of an European collection of HUS-associated EHEC, enabling to study properties and evolution of these important pathogens.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectGenotypeeng
dc.subjectMultilocus Sequence Typingeng
dc.subjectPhenotypeeng
dc.subjectPhylogenyeng
dc.subjectSeasonseng
dc.subjectVirulence/geneticseng
dc.subjectChild Preschooleng
dc.subjectInfanteng
dc.subjectSerotypingeng
dc.subjectHemolytic-Uremic Syndrome/epidemiologyeng
dc.subjectAnti-Bacterial Agents/pharmacologyeng
dc.subjectMicrobial Sensitivity Testseng
dc.subjectCzech Republic/epidemiologyeng
dc.subjectEnterohemorrhagic Escherichia coli/classificationeng
dc.subjectEnterohemorrhagic Escherichia coli/drug effectseng
dc.subjectEnterohemorrhagic Escherichia coli/geneticseng
dc.subjectEnterohemorrhagic Escherichia coli/isolation & purificationeng
dc.subjectHemolytic-Uremic Syndrome/diagnosiseng
dc.subjectHemolytic-Uremic Syndrome/microbiologyeng
dc.subjectShiga Toxin/geneticseng
dc.subject.ddc610 Medizin
dc.titleEnterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10032842
dc.identifier.doi10.1371/journal.pone.0073927
dc.identifier.doihttp://dx.doi.org/10.25646/1593
local.edoc.container-titlePLoS ONE
local.edoc.container-textMarejková M, Bláhová K, Janda J, Fruth A, Petráš P (2013) Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic. PLoS ONE 8(9): e73927.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073927
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume8
local.edoc.container-issue9
local.edoc.container-year2013

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