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2012-12-26Zeitschriftenartikel DOI: 10.1128/JVI.01116-12
Biological and Phylogenetic Characteristics of Yellow Fever Virus Lineages from West Africa
dc.contributor.authorStock, Nina K.
dc.contributor.authorLaraway, Hewád
dc.contributor.authorFaye, Ousmane
dc.contributor.authorDiallo, Mawlouth
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorSall, Amadou A.
dc.date.accessioned2018-05-07T17:11:47Z
dc.date.available2018-05-07T17:11:47Z
dc.date.created2013-10-31
dc.date.issued2012-12-26none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reRQIlOD3GyRI/PDF/23ZahLTeRRgic.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1722
dc.description.abstractThe yellow fever virus (YFV), the first proven human-pathogenic virus, although isolated in 1927, is still a major public health problem, especially in West Africa where it causes outbreaks every year. Nevertheless, little is known about its genetic diversity and evolutionary dynamics, mainly due to a limited number of genomic sequences from wild virus isolates. In this study, we analyzed the phylogenetic relationships of 24 full-length genomes from YFV strains isolated between 1973 and 2005 in a sylvatic context of West Africa, including 14 isolates that had previously not been sequenced. By this, we confirmed genetic variability within one genotype by the identification of various YF lineages circulating in West Africa. Further analyses of the biological properties of these lineages revealed differential growth behavior in human liver and insect cells, correlating with the source of isolation and suggesting host adaptation. For one lineage, repeatedly isolated in a context of vertical transmission, specific characteristics in the growth behavior and unique mutations of the viral genome were observed and deserve further investigation to gain insight into mechanisms involved in YFV emergence and maintenance in nature.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectAmino Acid Sequenceeng
dc.subjectMolecular Sequence Dataeng
dc.subjectGenotypeeng
dc.subjectPhylogenyeng
dc.subjectBase Sequenceeng
dc.subjectGenome Viraleng
dc.subjectLiver/virologyeng
dc.subjectViral Proteins/geneticseng
dc.subjectCell Lineeng
dc.subjectAedes/virologyeng
dc.subjectAfrica Westerneng
dc.subjectGenetic Variationeng
dc.subjectHep G2 Cellseng
dc.subjectInsect Vectors/virologyeng
dc.subjectRNA Viral/geneticseng
dc.subjectSequence Analysis RNAeng
dc.subjectViral Proteins/chemistryeng
dc.subjectYellow Fever/geneticseng
dc.subjectYellow Fever/virologyeng
dc.subjectYellow fever virus/classificationeng
dc.subjectYellow fever virus/geneticseng
dc.subjectYellow fever virus/isolation & purificationeng
dc.subject.ddc610 Medizin
dc.titleBiological and Phylogenetic Characteristics of Yellow Fever Virus Lineages from West Africa
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10033549
dc.identifier.doi10.1128/JVI.01116-12
dc.identifier.doihttp://dx.doi.org/10.25646/1647
local.edoc.container-titleJournal of Virology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://jvi.asm.org/content/87/5/2895.long
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume87
local.edoc.container-issue5
local.edoc.container-year2013

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