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2013-10-08Zeitschriftenartikel DOI: 10.1128/mBio.00601-13
The Novel Human Influenza A(H7N9) Virus Is Naturally Adapted to Efficient Growth in Human Lung Tissue
dc.contributor.authorKnepper, Jessica
dc.contributor.authorSchierhorn, Kristina L.
dc.contributor.authorBecher, Anne
dc.contributor.authorBudt, Matthias
dc.contributor.authorTönnies, Mario
dc.contributor.authorBauer, Torsten T.
dc.contributor.authorSchneider, Paul
dc.contributor.authorNeudecker, Jens
dc.contributor.authorRückert, Jens C.
dc.contributor.authorGruber, Achim D.
dc.contributor.authorSuttorp, Norbert
dc.contributor.authorSchweiger, Brunhilde
dc.contributor.authorHippenstiel, Stefan
dc.contributor.authorHocke, Andreas C.
dc.contributor.authorWolff, Thorsten
dc.date.accessioned2018-05-07T17:12:20Z
dc.date.available2018-05-07T17:12:20Z
dc.date.created2013-11-08
dc.date.issued2013-10-08none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rePxpQV6U97e2/PDF/21zcafKbwDv52.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1725
dc.description.abstractA novel influenza A virus (IAV) of the H7N9 subtype has been isolated from severely diseased patients with pneumonia and acute respiratory distress syndrome and, apparently, from healthy poultry in March 2013 in Eastern China. We evaluated replication, tropism, and cytokine induction of the A/Anhui/1/2013 (H7N9) virus isolated from a fatal human infection and two low-pathogenic avian H7 subtype viruses in a human lung organ culture system mimicking infection of the lower respiratory tract. The A(H7N9) patient isolate replicated similarly well as a seasonal IAV in explanted human lung tissue, whereas avian H7 subtype viruses propagated poorly. Interestingly, the avian H7 strains provoked a strong antiviral type I interferon (IFN-I) response, whereas the A(H7N9) virus induced only low IFN levels. Nevertheless, all viruses analyzed were detected predominantly in type II pneumocytes, indicating that the A(H7N9) virus does not differ in its cellular tropism from other avian or human influenza viruses. Tissue culture-based studies suggested that the low induction of the IFN-β promoter correlated with an efficient suppression by the viral NS1 protein. These findings demonstrate that the zoonotic A(H7N9) virus is unusually well adapted to efficient propagation in human alveolar tissue, which most likely contributes to the severity of lower respiratory tract disease seen in many patients.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectInfluenza Human/immunologyeng
dc.subjectInfluenza Human/pathologyeng
dc.subjectBirdseng
dc.subjectCell Lineeng
dc.subjectInfluenza Human/virologyeng
dc.subjectVirus Replicationeng
dc.subjectChinaeng
dc.subjectInfluenza A virus/growth & developmenteng
dc.subjectInfluenza A virus/isolation & purificationeng
dc.subjectInfluenza A virus/physiologyeng
dc.subjectInfluenza in Birds/virologyeng
dc.subjectInterferon-beta/immunologyeng
dc.subjectLung/immunologyeng
dc.subjectLung/pathologyeng
dc.subjectLung/virologyeng
dc.subject.ddc610 Medizin
dc.titleThe Novel Human Influenza A(H7N9) Virus Is Naturally Adapted to Efficient Growth in Human Lung Tissue
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10033636
dc.identifier.doi10.1128/mBio.00601-13
dc.identifier.doihttp://dx.doi.org/10.25646/1650
local.edoc.container-titlemBio
local.edoc.container-textKnepper J, Schierhorn KL, Becher A, Budt M, Tönnies M, Bauer TT, Schneider P, Neudecker J, Rückert JC, Gruber AD, Suttorp N, Schweiger B, Hippenstiel S, Hocke AC, Wolff T. 2013. The novel human influenza A(H7N9) virus is naturally adapted to efficient growth in human lung tissue. mBio 4(5):e00601-13.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://mbio.asm.org/content/4/5/e00601-13
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume4
local.edoc.container-issue5
local.edoc.container-year2013

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