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2013-07-12Zeitschriftenartikel DOI: 10.1128/JB.00754-13
DNA Uptake by the Nosocomial Pathogen Acinetobacter baumannii Occurs during Movement along Wet Surfaces
dc.contributor.authorWilharm, Gottfried
dc.contributor.authorPiesker, Janett
dc.contributor.authorLaue, Michael
dc.contributor.authorSkiebe, Evelyn
dc.date.accessioned2018-05-07T17:28:24Z
dc.date.available2018-05-07T17:28:24Z
dc.date.created2014-02-06
dc.date.issued2013-07-12none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reFNPlr6oZMYk/PDF/26GCt2gkk69RI.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1813
dc.description.abstractThe emergence of Acinetobacter baumannii as an increasingly multidrug-resistant nosocomial pathogen largely relies on acquisition of resistance genes via horizontal gene transfer. Here, we demonstrate that many clinical isolates of A. baumannii take up DNA while they move along wet surfaces. We show that both motility and DNA uptake are abolished after inactivation of pilT, which putatively encodes the type 4 pilus (T4P) retraction ATPase, and comEC, which putatively encodes the DNA uptake channel, respectively. Inactivation of pilT correlates with an increase in the number and length of pili with an average diameter of 7.2 nm. In the Galleria mellonella infection model, the comEC mutant is significantly attenuated, whereas the pilT mutant is not, dissecting biologically distinct roles of T4P and the DNA uptake channel. Collectively, these findings promote our understanding of the mechanisms of DNA uptake and resistance development in A. baumannii, which may also apply to other important pathogens.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionsepidemiologie
dc.subjectBacterial/geneticseng
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectTransformation Bacterialeng
dc.subjectDrug Resistance Multipleeng
dc.subjectBacterial Proteins/metabolismeng
dc.subjectDNA-Binding Proteins/geneticseng
dc.subjectDNA-Binding Proteins/metabolismeng
dc.subjectBacterial Proteins/geneticseng
dc.subjectAcinetobacter Infections/microbiologyeng
dc.subjectAcinetobacter baumannii/geneticseng
dc.subjectAcinetobacter baumannii/metabolismeng
dc.subjectAcinetobacter baumannii/pathogenicityeng
dc.subjectAcinetobacter baumannii/physiologyeng
dc.subjectAnti-Bacterial Agents/pharmacologyeng
dc.subjectCross Infection/microbiologyeng
dc.subjectDNA Bacterial/geneticseng
dc.subjectDNA Bacterial/metabolismeng
dc.subjectFimbriae Bacterial/drug effectseng
dc.subjectMoths/microbiologyeng
dc.subjectMovementeng
dc.subjectSurface Propertieseng
dc.subject.ddc610 Medizin
dc.titleDNA Uptake by the Nosocomial Pathogen Acinetobacter baumannii Occurs during Movement along Wet Surfaces
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10034864
dc.identifier.doi10.1128/JB.00754-13
dc.identifier.doihttp://dx.doi.org/10.25646/1738
local.edoc.container-titleJournal of Bacteriology
local.edoc.container-textWilharm, G., Piesker, J., Laue, M., Skiebe, E. DNA Uptake by the nosocomial pathogen acinetobacter baumannii occurs during movement along wet surfaces (2013) Journal of Bacteriology, 195 (18), pp. 4146-4153.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://jb.asm.org/content/195/18/4146
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume195
local.edoc.container-issue18
local.edoc.container-year2013

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