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2014-03-21Zeitschriftenartikel DOI: 10.1371/journal.pone.0092500
Quantitative Influenza Follow-Up Testing (QIFT)—A Novel Biomarker for the Monitoring of Disease Activity at the Point-of-Care
dc.contributor.authorChen, Xi
dc.contributor.authorYousef, Kaveh Pouran
dc.contributor.authorDuwe, Susanne
dc.contributor.authorKarsch, Katharina
dc.contributor.authorGrover, Sandeep
dc.contributor.authorWählisch, Stephanie
dc.contributor.authorObermeier, Patrick
dc.contributor.authorTief, Franziska
dc.contributor.authorMühlhans, Susann
dc.contributor.authorSeeber, Lea
dc.contributor.authorKleist, Max von
dc.contributor.authorSchweiger, Brunhilde
dc.contributor.authorRath, Barbara
dc.date.accessioned2018-05-07T17:36:35Z
dc.date.available2018-05-07T17:36:35Z
dc.date.created2014-04-07
dc.date.issued2014-03-21none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rei4lWPBTaRlY/PDF/20ZffyCMkn22.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1857
dc.description.abstractBackground: Influenza infections induce considerable disease burden in young children. Biomarkers for the monitoring of disease activity at the point-of-care (POC) are currently lacking. Recent methodologies for fluorescence-based rapid testing have been developed to provide improved sensitivities with the initial diagnosis. The present study aims to explore the utility of second-generation rapid testing during longitudinal follow-up of influenza patients (Rapid Influenza Follow-up Testing = RIFT). Signal/control fluorescent readouts (Quantitative Influenza Follow-up Testing = QIFT) are evaluated as a potential biomarker for the monitoring of disease activity at the POC. Methods and Findings: RIFT (SOFIA) and QIFT were performed at the POC and compared to blinded RT-PCR at the National Reference Centre for Influenza. From 10/2011-4/2013, a total of 2048 paediatric cases were studied prospectively; 273 cases were PCR-confirmed for influenza. During follow-up, RIFT results turned negative either prior to PCR (68%), or simultaneously (30%). The first negative RIFT occurred after a median of 8 days with a median virus load (VL) of 5.6×10∧3 copies/ml and cycle threshold of 37, with no evidence of viral rebound. Binning analysis revealed that QIFT differentiated accurately between patients with low, medium and high viral titres. QIFT increase/decrease showed 88% agreement (sensitivity = 52%, specificity = 95%) with VL increase/decrease, respectively. QIFT-based viral clearance estimates showed similar values compared to PCR-based estimates. Variations in viral clearance rates were lower in treated compared to untreated patients. The study was limited by use of non-invasive, semi-quantitative nasopharyngeal samples. VL measurements below the limit of detection could not be quantified reliably. Conclusions: During follow-up, RIFT provides a first surrogate measure for influenza disease activity. A “switch” from positive to negative values may indicate a drop in viral load below a critical threshold, where rebound is no longer expected. QIFT may provide a useful tool for the monitoring of disease burden and viral clearance at the POC.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subject.ddc610 Medizin
dc.titleQuantitative Influenza Follow-Up Testing (QIFT)—A Novel Biomarker for the Monitoring of Disease Activity at the Point-of-Care
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10036088
dc.identifier.doi10.1371/journal.pone.0092500
dc.identifier.doihttp://dx.doi.org/10.25646/1782
local.edoc.container-titlePLoS ONE
local.edoc.container-textChen X, Pouran Yousef K, Duwe S, Karsch K, Grover S, et al. (2014) Quantitative Influenza Follow-Up Testing (QIFT)—A Novel Biomarker for the Monitoring of Disease Activity at the Point-of-Care. PLoS ONE 9(3): e92500.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0092500
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume9
local.edoc.container-issue3
local.edoc.container-year2014

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