Zur Kurzanzeige

Reverse Transcription Recombinase Polymerase Amplification Assay for the Detection of Middle East Respiratory Syndrome Coronavirus
dc.contributor.authorWahed, Ahmed Abd El
dc.contributor.authorPatel, Pranav
dc.contributor.authorHeidenreich, Doris
dc.contributor.authorHufert, Frank T.
dc.contributor.authorWeidmann, Manfred
dc.date.accessioned2018-05-07T17:37:19Z
dc.date.available2018-05-07T17:37:19Z
dc.date.created2014-04-10
dc.date.issued2013-12-12none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re8RO1K03JTk/PDF/217VREL8MxU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1861
dc.description.abstractThe emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities. Currently, detection of MERS-CoV in patient samples is done by real-time RT-PCR. Samples collected from suspected cases are sent to highly-equipped centralized laboratories for screening. A rapid point-of-care test is needed to allow more widespread mobile detection of the virus directly from patient material. In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity. The isothermal (42°C) MERS-CoV RT-RPA was as sensitive as real-time RT-PCR (10 RNA molecules), rapid (3-7 minutes) and mobile (using tubescanner weighing 1kg). The MERS-CoV RT-RPA showed cross-detection neither of any of the RNAs of several coronaviruses and respiratory viruses affecting humans nor of the human genome. The developed isothermal real-time RT-RPA is ideal for rapid mobile molecular MERS-CoV monitoring in acute patients and may also facilitate the search for the animal reservoir of MERS-CoV.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectMERS-coronaviruseng
dc.subjectRPAeng
dc.subjectpoint-of-care assayeng
dc.subject.ddc610 Medizin
dc.titleReverse Transcription Recombinase Polymerase Amplification Assay for the Detection of Middle East Respiratory Syndrome Coronavirus
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10036141
dc.identifier.doi10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364
dc.identifier.doihttp://dx.doi.org/10.25646/1786
local.edoc.container-titlePLoS Current Outbreaks
local.edoc.container-textAbd El Wahed A, Patel P, Heidenreich D, Hufert FT, Weidmann M. Reverse Transcription Recombinase Polymerase Amplification Assay for the Detection of Middle East Respiratory Syndrome Coronavirus. PLOS Currents Outbreaks.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://currents.plos.org/outbreaks/article/reverse-transcription-recombinase-polymerase-amplification-assay-for-the-detection-of-middle-east-respiratory-syndrome-coronavirus/
local.edoc.container-publisher-namePublic Library of Science

Zur Kurzanzeige