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2014-04-21Zeitschriftenartikel DOI: 10.1371/journal.pone.0095379
Two Novel EHEC/EAEC Hybrid Strains Isolated from Human Infections
dc.contributor.authorPrager, Rita
dc.contributor.authorLang, Christina
dc.contributor.authorAurass, Philipp
dc.contributor.authorFruth, Angelika
dc.contributor.authorTietze, Erhard
dc.contributor.authorFlieger, Antje
dc.date.accessioned2018-05-07T17:39:00Z
dc.date.available2018-05-07T17:39:00Z
dc.date.created2014-05-12
dc.date.issued2014-04-21none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reRTZCAcVQQ/PDF/22H3RpSQQsO8o.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1870
dc.description.abstractThe so far highest number of life-threatening hemolytic uremic syndrome was associated with a food-borne outbreak in 2011 in Germany which was caused by an enterohemorrhagic Escherichia coli (EHEC) of the rare serotype O104:H4. Most importantly, the outbreak strain harbored genes characteristic of both EHEC and enteroaggregative E. coli (EAEC). Such strains have been described seldom but due to the combination of virulence genes show a high pathogenicity potential. To evaluate the importance of EHEC/EAEC hybrid strains in human disease, we analyzed the EHEC strain collection of the German National Reference Centre for Salmonella and other Bacterial Enteric Pathogens (NRC). After exclusion of O104:H4 EHEC/EAEC strains, out of about 2400 EHEC strains sent to NRC between 2008 and 2012, two strains exhibited both EHEC and EAEC marker genes, specifically were stx2 and aatA positive. Like the 2011 outbreak strain, one of the novel EHEC/EAEC harbored the Shiga toxin gene type stx2a. The strain was isolated from a patient with bloody diarrhea in 2010, was serotyped as O59:H−, belonged to MLST ST1136, and exhibited genes for type IV aggregative adherence fimbriae (AAF). The second strain was isolated from a patient with diarrhea in 2012, harbored stx2b, was typed as Orough:H−, and belonged to MLST ST26. Although the strain conferred the aggregative adherence phenotype, no known AAF genes corresponding to fimbrial types I to V were detected. In summary, EHEC/EAEC hybrid strains are currently rarely isolated from human disease cases in Germany and two novel EHEC/EAEC of rare serovars/MLST sequence types were characterized.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectGeneticeng
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectMolecular Sequence Dataeng
dc.subjectEscherichia coli/geneticseng
dc.subjectEscherichia coli Infections/microbiologyeng
dc.subjectPhenotypeeng
dc.subjectDisease Outbreakseng
dc.subjectEscherichia coli/isolation & purificationeng
dc.subjectElectrophoresis Gel Pulsed-Fieldeng
dc.subjectEnterohemorrhagic Escherichia coli/geneticseng
dc.subjectEnterohemorrhagic Escherichia coli/isolation & purificationeng
dc.subjectShiga Toxin/geneticseng
dc.subjectCercopithecus aethiopseng
dc.subjectDrug Resistanceeng
dc.subjectMicrobial/geneticseng
dc.subjectFimbriae Bacterial/metabolismeng
dc.subjectGenes Bacterialeng
dc.subjectHybridizationeng
dc.subjectPlasmids/metabolismeng
dc.subjectVero Cellseng
dc.subject.ddc610 Medizin
dc.titleTwo Novel EHEC/EAEC Hybrid Strains Isolated from Human Infections
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10036315
dc.identifier.doi10.1371/journal.pone.0095379
dc.identifier.doihttp://dx.doi.org/10.25646/1795
local.edoc.container-titlePLoS ONE
local.edoc.container-textPrager R, Lang C, Aurass P, Fruth A, Tietze E, et al. (2014) Two Novel EHEC/EAEC Hybrid Strains Isolated from Human Infections. PLoS ONE 9(4): e95379.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0095379
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume9
local.edoc.container-issue4
local.edoc.container-year2014

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