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2014-07-09Zeitschriftenartikel DOI: 10.1371/journal.pone.0099204
Beta-Lactam Antibiotics Stimulate Biofilm Formation in Non-Typeable Haemophilus influenzae by Up-Regulating Carbohydrate Metabolism
dc.contributor.authorWu, Siva
dc.contributor.authorLi, Xiaojin
dc.contributor.authorGunawardana, Manjula
dc.contributor.authorMaguire, Kathleen
dc.contributor.authorGuerrero-Given, Debbie
dc.contributor.authorSchaudinn, Christoph
dc.contributor.authorWang, Charles
dc.contributor.authorBaum, Marc M.
dc.contributor.authorWebster, Paul
dc.date.accessioned2018-05-07T17:49:10Z
dc.date.available2018-05-07T17:49:10Z
dc.date.created2014-08-07
dc.date.issued2014-07-09none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reuF2RmN2oFQ/PDF/28xos5mFEwSGU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1925
dc.description.abstractNon-typeable Haemophilus influenzae (NTHi) is a common acute otitis media pathogen, with an incidence that is increased by previous antibiotic treatment. NTHi is also an emerging causative agent of other chronic infections in humans, some linked to morbidity, and all of which impose substantial treatment costs. In this study we explore the possibility that antibiotic exposure may stimulate biofilm formation by NTHi bacteria. We discovered that sub-inhibitory concentrations of beta-lactam antibiotic (i.e., amounts that partially inhibit bacterial growth) stimulated the biofilm-forming ability of NTHi strains, an effect that was strain and antibiotic dependent. When exposed to sub-inhibitory concentrations of beta-lactam antibiotics NTHi strains produced tightly packed biofilms with decreased numbers of culturable bacteria but increased biomass. The ratio of protein per unit weight of biofilm decreased as a result of antibiotic exposure. Antibiotic-stimulated biofilms had altered ultrastructure, and genes involved in glycogen production and transporter function were up regulated in response to antibiotic exposure. Down-regulated genes were linked to multiple metabolic processes but not those involved in stress response. Antibiotic-stimulated biofilm bacteria were more resistant to a lethal dose (10 µg/mL) of cefuroxime. Our results suggest that beta-lactam antibiotic exposure may act as a signaling molecule that promotes transformation into the biofilm phenotype. Loss of viable bacteria, increase in biofilm biomass and decreased protein production coupled with a concomitant up-regulation of genes involved with glycogen production might result in a biofilm of sessile, metabolically inactive bacteria sustained by stored glycogen. These biofilms may protect surviving bacteria from subsequent antibiotic challenges, and act as a reservoir of viable bacteria once antibiotic exposure has ended.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectAdulteng
dc.subjectAgedeng
dc.subjectMiddle Agedeng
dc.subjectRisk Factorseng
dc.subjectYoung Adulteng
dc.subjectAnti-HIV Agents/therapeutic useeng
dc.subjectHIV Infections/epidemiologyeng
dc.subjectHIV Infections/virologyeng
dc.subjectPrevalenceeng
dc.subjectGenotypeeng
dc.subjectCluster Analysiseng
dc.subjectHIV-1/geneticseng
dc.subjectRetrospective Studieseng
dc.subjectHIV Infections/drug therapyeng
dc.subjectHIV-1/drug effectseng
dc.subjectPublic Health Surveillanceeng
dc.subjectMicrobial Sensitivity Testseng
dc.subjectHIV Infections/transmissioneng
dc.subjectDrug Resistance Viral/geneticseng
dc.subjectAnti-HIV Agents/pharmacologyeng
dc.subjectBelgium/epidemiologyeng
dc.subjectPregnancyeng
dc.subject.ddc610 Medizin
dc.titleBeta-Lactam Antibiotics Stimulate Biofilm Formation in Non-Typeable Haemophilus influenzae by Up-Regulating Carbohydrate Metabolism
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10037118
dc.identifier.doi10.1371/journal.pone.0099204
dc.identifier.doihttp://dx.doi.org/10.25646/1850
local.edoc.container-titlePLoS ONE
local.edoc.container-textWu S, Li X, Gunawardana M, Maguire K, Guerrero-Given D, et al. (2014) Beta- Lactam Antibiotics Stimulate Biofilm Formation in Non-Typeable Haemophilus influenzae by Up-Regulating Carbohydrate Metabolism. PLoS ONE 9(7): e99204.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0099204
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume9
local.edoc.container-issue7
local.edoc.container-year2014

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