Show simple item record

2014-07-11Zeitschriftenartikel DOI: 10.1186/1756-3305-7-321
Epidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization
dc.contributor.authorHelmy, Yosra A.
dc.contributor.authorKlotz, Christian
dc.contributor.authorWilking, Hendrik
dc.contributor.authorKrücken, Jürgen
dc.contributor.authorNöckler, Karsten
dc.contributor.authorSamson-Himmelstjerna, Georg von
dc.contributor.authorZessin, Karl-H.
dc.contributor.authorAebischer, Toni
dc.date.accessioned2018-05-07T17:51:04Z
dc.date.available2018-05-07T17:51:04Z
dc.date.created2014-08-15
dc.date.issued2014-07-11none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/ret3cqVajEheM/PDF/27zLdz5szYP3U.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1935
dc.description.abstractBackground: Giardia duodenalis is a common flagellated protozoan parasite that infects the small intestine of a wide range of vertebrate hosts. This study aimed to determine whether tracing of G. duodenalis isolates by current genetic typing tools is possible using an exemplary set of samples from infected cattle, buffalo and children from the Ismailia province, Egypt. Method: A total of 804 fecal samples from ruminant animals was collected from 191 herds and 165 samples from diarrheal children below the age of 10 years. Parasites were detected in these samples using the copro-antigen RIDA®QUICK test and by real-time PCR. Samples were then genetically characterized based on the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes. Results: The prevalence of G. duodenalis was 53% in ruminants and 21% in symptomatic children and infection was not positively correlated with diarrheal symptoms. Sequence typing analysis confirmed predominance of B-type sequences (>67%) in humans and E-type sequences (>81%) in ruminants over A-type sequences. For 39 samples the complete sequence information of the three marker gene fragments could be derived. Integration of the concatenated sequence information of the three marker gene fragments with the spatial data of the respective sample revealed that identical or near identical (only up to 1 out of 1358 bp different) concatenated sequencing types were spatially related in 4 out of 5 cases. Conclusion: The risk of zoonotic infection emanating from ruminants even in high prevalence areas is negligible. Genetic characterization indicated a predominant anthropogenic cycle of infection within the pediatric population studied. Integration of sequence typing data with information on geographic origins of samples allows parasite sub-population tracing using current typing tools.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionsepidemiologie
dc.subjectGiardiaeng
dc.subjectGenetic characterizationeng
dc.subjectRuminantseng
dc.subjectDiarrheal childreneng
dc.subjectReal time PCReng
dc.subjectRIDA®QUICK testeng
dc.subjectTriose phosphate isomerase (tpi)eng
dc.subjectGlutamate dehydrogenase (gdh)eng
dc.subjectβ-giardin (bg)eng
dc.subjectSpatial distributioneng
dc.subjectEgypteng
dc.subject.ddc610 Medizin
dc.titleEpidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10037228
dc.identifier.doi10.1186/1756-3305-7-321
dc.identifier.doihttp://dx.doi.org/10.25646/1860
local.edoc.container-titleParasites & Vectors
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.parasitesandvectors.com/content/7/1/321
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume7
local.edoc.container-issue321
local.edoc.container-year2014

Show simple item record