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2012-04-23Zeitschriftenartikel DOI: 10.1016/j.ijmm.2012.02.001
The mglA gene and its flanking regions in Brucella: The role of mglA in tolerance to hostile environments, Fe-metabolism and in vivo persistence
dc.contributor.authorJacob, Jens
dc.contributor.authorFinke, Antje
dc.contributor.authorMielke, Martin
dc.date.accessioned2018-05-07T17:58:10Z
dc.date.available2018-05-07T17:58:10Z
dc.date.created2014-10-10
dc.date.issued2012-04-23none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reldstyVksaWU/PDF/26x2GNxnsyQQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1973
dc.description.abstractWe previously demonstrated that a spontaneous smooth small-colony variant of Brucella abortus S19 is characterized by increased in vivo persistence and the differential expression of a gene predicted to encode a galactoside transport ATP binding protein (mglA). In order to further investigate the role of this gene in the context of its flanking regions, we analyzed the respective DNA sequences from the formerly described B. abortus S19 as well as from avirulent B. neotomae 5K33 and compared these with published data from other Brucella species. Deletion mutagenesis of mglA in the large-colony variant of B. abortus S19 resulted in increased tolerance of the deletion mutant to a hyperosmotic (toxic), galactose-containing medium as well as to oxidative stress (H2O2). Whilst the deletion mutant is characterized by reduced growth on solid Fe3+-containing minimal medium (small-colony morphology), in vivo studies in mice demonstrated statistical significant differences in the bacterial load of spleens in the pre-immune, but not in the late phase of the infection.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectBacterial/geneticseng
dc.subjectAnimalseng
dc.subjectFemaleeng
dc.subjectMolecular Sequence Dataeng
dc.subjectMiceeng
dc.subjectGene Deletioneng
dc.subjectDNAeng
dc.subjectSequence Analysis DNAeng
dc.subjectDisease Models Animaleng
dc.subjectCulture Media/chemistryeng
dc.subjectMice Inbred BALB Ceng
dc.subjectVirulenceeng
dc.subjectBacterial Loadeng
dc.subjectBrucella/geneticseng
dc.subjectBrucella/pathogenicityeng
dc.subjectBrucella/physiologyeng
dc.subjectBrucellosis/microbiologyeng
dc.subjectIron/metabolismeng
dc.subjectOsmotic Pressureeng
dc.subjectOxidative Stresseng
dc.subjectSpleen/microbiologyeng
dc.subjectStress Physiologicaleng
dc.subject.ddc610 Medizin
dc.titleThe mglA gene and its flanking regions in Brucella: The role of mglA in tolerance to hostile environments, Fe-metabolism and in vivo persistence
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10037796
dc.identifier.doi10.1016/j.ijmm.2012.02.001
dc.identifier.doihttp://dx.doi.org/10.25646/1898
local.edoc.container-titleInternational Journal of Medical Microbiology
local.edoc.container-textJacob, J., Finke, A., Mielke, M. The mglA gene and its flanking regions in Brucella: The role of mglA in tolerance to hostile environments, Fe-metabolism and in vivo persistence (2012) International Journal of Medical Microbiology, 302 (3), pp. 148-154.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S1438422112000124
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume302
local.edoc.container-issue3
local.edoc.container-year2012

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