Protocol for Metagenomic Virus Detection in Clinical Specimens
dc.contributor.author | Kohl, Claudia | |
dc.contributor.author | Brinkmann, Annika | |
dc.contributor.author | Dabrowski, Piotr Wojtek | |
dc.contributor.author | Radonić, Aleksandar | |
dc.contributor.author | Nitsche, Andreas | |
dc.contributor.author | Kurth, Andreas | |
dc.date.accessioned | 2018-05-07T18:01:50Z | |
dc.date.available | 2018-05-07T18:01:50Z | |
dc.date.created | 2015-01-05 | |
dc.date.issued | 2014-12-15 | none |
dc.identifier.other | http://edoc.rki.de/oa/articles/rektSWzC3z8tk/PDF/28zM4vvcwqf.pdf | |
dc.identifier.uri | http://edoc.rki.de/176904/1993 | |
dc.description.abstract | Sixty percent of emerging viruses have a zoonotic origin, making transmission from animals a major threat to public health. Prompt identification and analysis of these pathogens are indispensable to taking action toward prevention and protection of the affected population. We quantifiably compared classical and modern approaches of virus purification and enrichment in theory and experiments. Eventually, we established an unbiased protocol for detection of known and novel emerging viruses from organ tissues (tissue-based universal virus detection for viral metagenomics [TUViD-VM]). The final TUViD-VM protocol was extensively validated by using real-time PCR and next-generation sequencing. We could increase the amount of detectable virus nucleic acids and improved the detection of viruses | eng |
dc.language.iso | eng | |
dc.publisher | Robert Koch-Institut, Biologische Sicherheit | |
dc.subject | Viral | eng |
dc.subject | Animals | eng |
dc.subject | Humans | eng |
dc.subject | Real-Time Polymerase Chain Reaction | eng |
dc.subject | DNA Viral/genetics | eng |
dc.subject | High-Throughput Nucleotide Sequencing | eng |
dc.subject | Molecular Diagnostic Techniques | eng |
dc.subject | Callithrix | eng |
dc.subject | Chick Embryo | eng |
dc.subject | DNA Viral/isolation & purification | eng |
dc.subject | Genes | eng |
dc.subject | Metagenome | eng |
dc.subject | Signal-To-Noise Ratio | eng |
dc.subject | Virus Diseases/diagnosis | eng |
dc.subject | Zoonoses | eng |
dc.subject.ddc | 610 Medizin | |
dc.title | Protocol for Metagenomic Virus Detection in Clinical Specimens | |
dc.type | periodicalPart | |
dc.identifier.urn | urn:nbn:de:0257-10038257 | |
dc.identifier.doi | 10.3201/eid2101.140766 | |
dc.identifier.doi | http://dx.doi.org/10.25646/1918 | |
local.edoc.container-title | Emerging Infectious Diseases | |
local.edoc.fp-subtype | Artikel | |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-url | http://wwwnc.cdc.gov/eid/article/21/1/14-0766_article | |
local.edoc.container-publisher-name | Centers for Disease Control and Prevention | |
local.edoc.container-volume | 21 | |
local.edoc.container-issue | 1 | |
local.edoc.container-year | 2015 |