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2014-12-15Zeitschriftenartikel DOI: 10.3201/eid2101.140766
Protocol for Metagenomic Virus Detection in Clinical Specimens
dc.contributor.authorKohl, Claudia
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorDabrowski, Piotr Wojtek
dc.contributor.authorRadonić, Aleksandar
dc.contributor.authorNitsche, Andreas
dc.contributor.authorKurth, Andreas
dc.date.accessioned2018-05-07T18:01:50Z
dc.date.available2018-05-07T18:01:50Z
dc.date.created2015-01-05
dc.date.issued2014-12-15none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rektSWzC3z8tk/PDF/28zM4vvcwqf.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1993
dc.description.abstractSixty percent of emerging viruses have a zoonotic origin, making transmission from animals a major threat to public health. Prompt identification and analysis of these pathogens are indispensable to taking action toward prevention and protection of the affected population. We quantifiably compared classical and modern approaches of virus purification and enrichment in theory and experiments. Eventually, we established an unbiased protocol for detection of known and novel emerging viruses from organ tissues (tissue-based universal virus detection for viral metagenomics [TUViD-VM]). The final TUViD-VM protocol was extensively validated by using real-time PCR and next-generation sequencing. We could increase the amount of detectable virus nucleic acids and improved the detection of viruseseng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectViraleng
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectReal-Time Polymerase Chain Reactioneng
dc.subjectDNA Viral/geneticseng
dc.subjectHigh-Throughput Nucleotide Sequencingeng
dc.subjectMolecular Diagnostic Techniqueseng
dc.subjectCallithrixeng
dc.subjectChick Embryoeng
dc.subjectDNA Viral/isolation & purificationeng
dc.subjectGeneseng
dc.subjectMetagenomeeng
dc.subjectSignal-To-Noise Ratioeng
dc.subjectVirus Diseases/diagnosiseng
dc.subjectZoonoseseng
dc.subject.ddc610 Medizin
dc.titleProtocol for Metagenomic Virus Detection in Clinical Specimens
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10038257
dc.identifier.doi10.3201/eid2101.140766
dc.identifier.doihttp://dx.doi.org/10.25646/1918
local.edoc.container-titleEmerging Infectious Diseases
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://wwwnc.cdc.gov/eid/article/21/1/14-0766_article
local.edoc.container-publisher-nameCenters for Disease Control and Prevention
local.edoc.container-volume21
local.edoc.container-issue1
local.edoc.container-year2015

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