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2015-06-01Zeitschriftenartikel DOI: 10.1371/journal.pone.0128349
Outbreak of Uncommon O4 Non-Agglutinating Salmonella Typhimurium Linked to Minced Pork, Saxony-Anhalt, Germany, January to April 2013
dc.contributor.authorAlt, Katja
dc.contributor.authorSimon, Sandra
dc.contributor.authorHelmeke, Carina
dc.contributor.authorKohlstock, Claudia
dc.contributor.authorPrager, Rita
dc.contributor.authorTietze, Erhard
dc.contributor.authorRabsch, Wolfgang
dc.contributor.authorKaragiannis, Ioannis
dc.contributor.authorWerber, Dirk
dc.contributor.authorFrank, Christina
dc.contributor.authorFruth, Angelika
dc.date.accessioned2018-05-07T18:15:51Z
dc.date.available2018-05-07T18:15:51Z
dc.date.created2015-06-05
dc.date.issued2015-06-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reIUQ6PLXAbo/PDF/22yMjHafbdtk.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2068
dc.description.abstractIntroduction: In January 2013, the National Reference Centre for Salmonella (NRC) detected a salmonellosis cluster in Saxony-Anhalt, Germany, caused by uncommon O4 non-agglutinating, monophasic Salmonella (S.) Typhimurium DT193. Circulating predominant monophasic S. Typhimurium DT193 clones typically display resistance phenotype ASSuT. We investigated common exposures to control the outbreak, and conducted microbiological investigations to assess the strains’ phenotype. Methods: We conducted a case-control study defining cases as persons living or working in Saxony-Anhalt diagnosed with the O4 non-agglutinating strain between January and March 2013. We selected two controls contemporarily reported with norovirus infection, frequency-matched on residence and age group, per case. We interviewed regarding food consumption, especially pork and its place of purchase. We calculated odds ratios (ORs) with 95% confidence intervals (95% CI) using logistic regression. The NRC investigated human and food isolates by PCR, SDS-PAGE, MLST, PFGE, MLVA and susceptibility testing. Results: Altogether, 68 O4 non-agglutinating human isolates were confirmed between January and April 2013. Of those, 61 were assigned to the outbreak (median age 57 years, 44% female); 83% cases ≥ 60 years were hospitalized. Eating raw minced pork from butcheries within 3 days was associated with disease (31 cases, 28 controls; OR adjusted for sex: 3.6; 95% CI: 1.0-13). Phage type DT193 and MLST ST34 were assigned, and isolates’ lipopolysaccharide (LPS) matched control strains. Isolates linked to Saxony-Anhalt exhibited PFGE type 5. ASSuT- and ACSSuT phenotype proportions were 34 and 39% respectively; 54% were resistant to chloramphenicol. Three pork isolates matched the outbreak strain. Discussion: Raw minced pork was the most likely infection vehicle in this first reported outbreak caused by O4 non-agglutinating, mostly chloramphenicol-resistant S. Typhimurium DT193. High hospitalization proportions demand awareness on the risk of consumption of raw pork among elderly. LPS analysis indicated O4 expression; therefore, testing with antisera from different lots is recommendable in unexpected agglutination reactions.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger; Robert Koch-Institut, Infektionsepidemiologie
dc.subjectHumanseng
dc.subjectMaleeng
dc.subjectAdulteng
dc.subjectAgedeng
dc.subjectGermany/epidemiologyeng
dc.subjectMiddle Agedeng
dc.subjectYoung Adulteng
dc.subjectPhenotypeeng
dc.subjectDisease Outbreakseng
dc.subjectCase-Control Studieseng
dc.subjectSalmonella Food Poisoning/epidemiologyeng
dc.subjectSalmonella Food Poisoning/microbiologyeng
dc.subjectFood Handlingeng
dc.subjectFood Safetyeng
dc.subjectRed Meat/microbiologyeng
dc.subjectSalmonella typhimurium/isolation & purificationeng
dc.subjectSalmonella typhimurium/physiologyeng
dc.subject.ddc610 Medizin
dc.titleOutbreak of Uncommon O4 Non-Agglutinating Salmonella Typhimurium Linked to Minced Pork, Saxony-Anhalt, Germany, January to April 2013
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10039572
dc.identifier.doi10.1371/journal.pone.0128349
dc.identifier.doihttp://dx.doi.org/10.25646/1993
local.edoc.container-titlePLoS ONE
local.edoc.container-textAlt K, Simon S, Helmeke C, Kohlstock C, Prager R, Tietze E, et al. (2015) Outbreak of Uncommon O4 Non-Agglutinating Salmonella Typhimurium Linked to Minced Pork, Saxony-Anhalt, Germany, January to April 2013. PLoS ONE 10(6): e0128349.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128349
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume10
local.edoc.container-issue6
local.edoc.container-year2015

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