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2007-10-24Zeitschriftenartikel DOI: 10.1186/1743-422X-4-105
Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein
dc.contributor.authorDiCarlo, Andrea
dc.contributor.authorMöller, Peggy
dc.contributor.authorLander, Angelika
dc.contributor.authorKolesnikova, Larissa
dc.contributor.authorBecker, Stephan
dc.date.accessioned2018-05-07T18:17:20Z
dc.date.available2018-05-07T18:17:20Z
dc.date.created2015-06-09
dc.date.issued2007-10-24none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reXQJaA67x4zE/PDF/26OnkJBMNyYsc.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2076
dc.description.abstractThe nucleoprotein (NP) of Marburg virus (MARV) is responsible for the encapsidation of viral genomic RNA and the formation of the helical nucleocapsid precursors that accumulate in intracellular inclusions in infected cells. To form the large helical MARV nucleocapsid, NP needs to interact with itself and the viral proteins VP30, VP35 and L, which are also part of the MARV nucleocapsid. In the present study, a conserved coiled coil motif in the central part of MARV NP was shown to be an important element for the interactions of NP with itself and VP35, the viral polymerase cofactor. Additionally, the coiled coil motif was essential for the formation of NP-induced intracellular inclusions and for the function of NP in the process of transcription and replication of viral RNA in a minigenome system. Transfer of the coiled coil motif to a reporter protein was sufficient to mediate interaction of the constructed fusion protein with the N-terminus of NP. The coiled coil motif is bipartite, constituted by two coiled coils which are separated by a flexible linker.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectRNA Viral/geneticseng
dc.subjectConserved Sequenceeng
dc.subjectHeLa Cellseng
dc.subjectMarburgvirus/geneticseng
dc.subjectMarburgvirus/physiologyeng
dc.subjectNucleocapsid Proteins/chemistryeng
dc.subjectNucleocapsid Proteins/physiologyeng
dc.subjectNucleoproteins/chemistryeng
dc.subjectNucleoproteins/physiologyeng
dc.subjectRNA Viral/biosynthesiseng
dc.subjectTranscription Geneticeng
dc.subjectViral Proteins/physiologyeng
dc.subject.ddc610 Medizin
dc.titleNucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10039676
dc.identifier.doi10.1186/1743-422X-4-105
dc.identifier.doihttp://dx.doi.org/10.25646/2001
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/4/1/105
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume4
local.edoc.container-issue105
local.edoc.container-year2007

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