Show simple item record

2015-06-04Zeitschriftenartikel DOI: 10.1371/journal.ppat.1004883
The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
dc.contributor.authorAeberhard, Lukas
dc.contributor.authorBanhart, Sebastian
dc.contributor.authorFischer, Martina
dc.contributor.authorJehmlich, Nico
dc.contributor.authorRose, Laura
dc.contributor.authorKoch, Sophia
dc.contributor.authorLaue, Michael
dc.contributor.authorRenard, Bernhard Y.
dc.contributor.authorSchmidt, Frank
dc.contributor.authorHeuer, Dagmar
dc.date.accessioned2018-05-07T18:18:39Z
dc.date.available2018-05-07T18:18:39Z
dc.date.created2015-06-30
dc.date.issued2015-06-04none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reCXTYKiV9gXg/PDF/27Kq1zF3U9aTM.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2083
dc.description.abstractChlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. Computational analysis indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells’ antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular, SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, these findings suggest that C. trachomatis hijacks retrograde pathways for effective infection.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectHumanseng
dc.subjectProteomicseng
dc.subjectHeLa Cellseng
dc.subjectCell Separationeng
dc.subjectChlamydia Infections/metabolismeng
dc.subjectChlamydia trachomatis/metabolismeng
dc.subjectChlamydia trachomatis/pathogenicityeng
dc.subjectFlow Cytometryeng
dc.subjectInclusion Bodies/metabolismeng
dc.subjectProtein Transporteng
dc.subjectProteomeeng
dc.subjectRNA Small Interferingeng
dc.subjectTandem Mass Spectrometryeng
dc.subjectTransfectioneng
dc.subjectVacuoles/metabolismeng
dc.subject.ddc610 Medizin
dc.titleThe Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10039834
dc.identifier.doi10.1371/journal.ppat.1004883
dc.identifier.doihttp://dx.doi.org/10.25646/2008
local.edoc.container-titlePLoS Pathogens
local.edoc.container-textAeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, et al. (2015) The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog 11(6): e1004883.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1004883
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume11
local.edoc.container-issue6
local.edoc.container-year2015

Show simple item record