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2015-11-02Zeitschriftenartikel DOI: 10.1186/s12989-015-0108-2
Proteomic analysis of protein carbonylation: a useful tool to unravel nanoparticle toxicity mechanisms
dc.contributor.authorDriessen, Marc D.
dc.contributor.authorMues, Sarah
dc.contributor.authorVennemann, Antje
dc.contributor.authorHellack, Bryan
dc.contributor.authorBannuscher, Anne
dc.contributor.authorVimalakanthan, Vishalini
dc.contributor.authorRiebeling, Christian
dc.contributor.authorOssig, Rainer
dc.contributor.authorWiemann, Martin
dc.contributor.authorSchnekenburger, Jürgen
dc.contributor.authorKuhlbusch, Thomas A. J.
dc.contributor.authorRenard, Bernhard Y.
dc.contributor.authorLuch, Andreas
dc.contributor.authorHaase, Andrea
dc.date.accessioned2018-05-07T18:34:19Z
dc.date.available2018-05-07T18:34:19Z
dc.date.created2015-11-12
dc.date.issued2015-11-02none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reXaw7R3sKdmk/PDF/28Sx6rgN34Ck.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2167
dc.description.abstractBackground: Oxidative stress, a commonly used paradigm to explain nanoparticle (NP)-induced toxicity, results from an imbalance between reactive oxygen species (ROS) generation and detoxification. As one consequence, protein carbonyl levels may become enhanced. Thus, the qualitative and quantitative description of protein carbonylation may be used to characterize how biological systems respond to oxidative stress induced by NPs. Methods: We investigated a representative panel of 24 NPs including functionalized amorphous silica (6), zirconium dioxide (4), silver (4), titanium dioxide (3), zinc oxide (2), multiwalled carbon nanotubes (3), barium sulfate and boehmite. Surface reactivities of all NPs were studied in a cell-free system by electron spin resonance (ESR). NRK-52E cells were treated with all NPs, analyzed for viability (WST-1 assay) and intracellular ROS production (DCFDA assay). Carbonylated proteins were assessed by 1D and/or 2D immunoblotting and identified by matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/TOF). In parallel, tissue homogenates from rat lungs intratracheally instilled with silver NPs were studied. Results: Eleven NPs induced elevated levels of carbonylated proteins. This was in good agreement with the surface reactivity of the NPs as obtained by ESR and the reduction in cell viability as assessed by WST-1 assay. By contrast, results obtained by DCFDA assay were deviating. Each NP induced an individual pattern of protein carbonyls on 2D immunoblots. Affected proteins comprised cytoskeletal components, proteins being involved in stress response, or cytoplasmic enzymes of central metabolic pathways such as glycolysis and gluconeogenesis. Furthermore, induction of carbonyls upon silver NP treatment was also verified in rat lung tissue homogenates. Conclusions: Analysis of protein carbonylation is a versatile and sensitive method to describe NP-induced oxidative stress and, therefore, can be used to identify NPs of concern. Furthermore, detailed information about compromised proteins may aid in classifying NPs according to their mode of action.ger
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectSurface functionalizationeng
dc.subjectProtein carbonylationeng
dc.subjectOxidative stresseng
dc.subjectSilica nanoparticleseng
dc.subjectZirconium oxide nanoparticleseng
dc.subjectSilver nanoparticleseng
dc.subjectRat lungeng
dc.subjectESReng
dc.subjectDCFDAeng
dc.subject.ddc610 Medizin
dc.titleProteomic analysis of protein carbonylation: a useful tool to unravel nanoparticle toxicity mechanisms
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10041498
dc.identifier.doi10.1186/s12989-015-0108-2
dc.identifier.doihttp://dx.doi.org/10.25646/2092
local.edoc.container-titleParticle and Fibre Toxicology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-015-0108-2
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume12
local.edoc.container-issue36
local.edoc.container-year2015

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