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2015-11-10Zeitschriftenartikel DOI: 10.1371/journal.pone.0141527
Comparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the Species- and Strain-Specific Proteome
dc.contributor.authorDöllinger, Jörg
dc.contributor.authorSchaade, Lars
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2018-05-07T18:34:53Z
dc.date.available2018-05-07T18:34:53Z
dc.date.created2015-11-20
dc.date.issued2015-11-10none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reuGGuSILEMvg/PDF/24OeXJhz9M0DI.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2170
dc.description.abstractCowpox virus (CPXV) causes most zoonotic orthopoxvirus (OPV) infections in Europe and Northern as well as Central Asia. The virus has the broadest host range of OPV and is transmitted to humans from rodents and other wild or domestic animals. Increasing numbers of human CPXV infections in a population with declining immunity have raised concerns about the virus’ zoonotic potential. While there have been reports on the proteome of other human-pathogenic OPV, namely vaccinia virus (VACV) and monkeypox virus (MPXV), the protein composition of the CPXV mature virion (MV) is unknown. This study focused on the comparative analysis of the VACV and CPXV MV proteome by label-free single-run proteomics using nano liquid chromatography and high-resolution tandem mass spectrometry (nLC-MS/MS). The presented data reveal that the common VACV and CPXV MV proteome contains most of the known conserved and essential OPV proteins and is associated with cellular proteins known to be essential for viral replication. While the species-specific proteome could be linked mainly to less genetically-conserved gene products, the strain-specific protein abundance was found to be of high variance in proteins associated with entry, host-virus interaction and protein processing.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectSpecies Specificityeng
dc.subjectViral Proteins/geneticseng
dc.subjectCapsid Proteins/geneticseng
dc.subjectVirus Replicationeng
dc.subjectGenes Viraleng
dc.subjectDNA Viral/geneticseng
dc.subjectProteomeeng
dc.subjectCapsid Proteins/analysiseng
dc.subjectChromatography Liquid/methodseng
dc.subjectCowpox virus/chemistryeng
dc.subjectCowpox virus/geneticseng
dc.subjectCowpox virus/physiologyeng
dc.subjectNanotechnology/methodseng
dc.subjectTandem Mass Spectrometry/methodseng
dc.subjectVaccinia virus/chemistryeng
dc.subjectVaccinia virus/geneticseng
dc.subjectVaccinia virus/physiologyeng
dc.subjectViral Plaque Assayeng
dc.subjectViral Proteins/analysiseng
dc.subjectVirion/chemistryeng
dc.subjectVirion/isolation & purificationeng
dc.subject.ddc610 Medizin
dc.titleComparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the Species- and Strain-Specific Proteome
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10041560
dc.identifier.doi10.1371/journal.pone.0141527
dc.identifier.doihttp://dx.doi.org/10.25646/2095
local.edoc.container-titlePLoS ONE
local.edoc.container-textDoellinger J, Schaade L, Nitsche A (2015) Comparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the Species- and Strain-Specific Proteome. PLoS ONE 10(11): e0141527.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141527
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume10
local.edoc.container-issue11
local.edoc.container-year2015

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