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2015-07-31Zeitschriftenartikel DOI: 10.1128/AEM.01921-15
Highly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC
dc.contributor.authorEichhorn, Inga
dc.contributor.authorHeidemanns, Katrin
dc.contributor.authorSemmler, Torsten
dc.contributor.authorKinnemann, Bianca
dc.contributor.authorMellmann, Alexander
dc.contributor.authorHarmsen, Dag
dc.contributor.authorAnjum, Muna F.
dc.contributor.authorSchmidt, Herbert
dc.contributor.authorFruth, Angelika
dc.contributor.authorValentin-Weigand, Peter
dc.contributor.authorHeesemann, Jürgen
dc.contributor.authorSuerbaum, Sebastian
dc.contributor.authorKarch, Helge
dc.contributor.authorWieler, Lothar H.
dc.date.accessioned2018-05-07T18:42:58Z
dc.date.available2018-05-07T18:42:58Z
dc.date.created2015-12-21
dc.date.issued2015-07-31none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reRA92eIYa4OM/PDF/23bJXQs9MI7D2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2214
dc.description.abstractEnterohemorrhagic Escherichia coli (EHEC) is the causative agent of bloody diarrhea and extraintestinal sequelae in humans, most importantly hemolytic-uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Besides the bacteriophage-encoded Shiga toxin gene (stx), EHEC harbors the locus of enterocyte effacement (LEE), which confers the ability to cause attaching and effacing lesions. Currently, the vast majority of EHEC infections are caused by strains belonging to five O serogroups (the “big five”), which, in addition to O157, the most important, comprise O26, O103, O111, and O145. We hypothesize that these four non-O157 EHEC serotypes differ in their phylogenies. To test this hypothesis, we used multilocus sequence typing (MLST) to analyze a large collection of 250 isolates of these four O serogroups, which were isolated from diseased as well as healthy humans and cattle between 1952 and 2009. The majority of the EHEC isolates of O serogroups O26 and O111 clustered into one sequence type complex, STC29. Isolates of O103 clustered mainly in STC20, and most isolates of O145 were found within STC32. In addition to these EHEC strains, STC29 also included stx-negative E. coli strains, termed atypical enteropathogenic E. coli (aEPEC), yet another intestinal pathogenic E. coli group. The finding that aEPEC and EHEC isolates of non-O157 O serogroups share the same phylogeny suggests an ongoing microevolutionary scenario in which the phage-encoded Shiga toxin gene stx is transferred between aEPEC and EHEC. As a consequence, aEPEC strains of STC29 can be regarded as post- or pre-EHEC isolates. Therefore, STC29 incorporates phylogenetic information useful for unraveling the evolution of EHEC.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionsepidemiologie
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectCattleeng
dc.subjectEscherichia coli Infections/microbiologyeng
dc.subjectGenotypeeng
dc.subjectMultilocus Sequence Typingeng
dc.subjectPhylogenyeng
dc.subjectCluster Analysiseng
dc.subjectEvolution Moleculareng
dc.subjectEnterohemorrhagic Escherichia coli/classificationeng
dc.subjectEnterohemorrhagic Escherichia coli/geneticseng
dc.subjectEnterohemorrhagic Escherichia coli/isolation & purificationeng
dc.subjectSerogroupeng
dc.subjectColiphages/geneticseng
dc.subjectEnteropathogenic Escherichia coli/classificationeng
dc.subjectEnteropathogenic Escherichia coli/geneticseng
dc.subjectEnteropathogenic Escherichia coli/isolation & purificationeng
dc.subjectEscherichia coli Infections/veterinaryeng
dc.subjectHealthy Volunteerseng
dc.subjectShiga Toxins/geneticseng
dc.subject.ddc610 Medizin
dc.titleHighly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10042365
dc.identifier.doi10.1128/AEM.01921-15
dc.identifier.doihttp://dx.doi.org/10.25646/2139
local.edoc.container-titleApplied and Environmental Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://aem.asm.org/content/81/20/7041
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume81
local.edoc.container-issue20
local.edoc.container-year2015

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