Zur Kurzanzeige

2015-11-26Zeitschriftenartikel DOI: 10.3390/toxins7124859
An International Proficiency Test to Detect, Identify and Quantify Ricin in Complex Matrices
dc.contributor.authorWorbs, Sylvia
dc.contributor.authorSkiba, Martin
dc.contributor.authorBender, Jennifer K.
dc.contributor.authorZeleny, Reinhard
dc.contributor.authorSchimmel, Heinz
dc.contributor.authorLuginbühl, Werner
dc.contributor.authorDorner, Brigitte
dc.date.accessioned2018-05-07T18:44:05Z
dc.date.available2018-05-07T18:44:05Z
dc.date.created2015-12-21
dc.date.issued2015-11-26none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rerHMRSzcdU2/PDF/210Lp1CHskTIY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2220
dc.description.abstractWhile natural intoxications with seeds of Ricinus communis (R. communis) have long been known, the toxic protein ricin contained in the seeds is of major concern since it attracts attention of those intending criminal, terroristic and military misuse. In order to harmonize detection capabilities in expert laboratories, an international proficiency test was organized that aimed at identifying good analytical practices (qualitative measurements) and determining a consensus concentration on a highly pure ricin reference material (quantitative measurements). Sample materials included highly pure ricin as well as the related R. communis agglutinin (RCA120) spiked into buffer, milk and meat extract; additionally, an organic fertilizer naturally contaminated with R. communis shred was investigated in the proficiency test. The qualitative results showed that either a suitable combination of immunological, mass spectrometry (MS)-based and functional approaches or sophisticated MS-based approaches alone successfully allowed the detection and identification of ricin in all samples. In terms of quantification, it was possible to determine a consensus concentration of the highly pure ricin reference material. The results provide a basis for further steps in quality assurance and improve biopreparedness in expert laboratories worldwide.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectricineng
dc.subjectproficiency testeng
dc.subjectreference materialeng
dc.subjectstandardized detectioneng
dc.subject.ddc610 Medizin
dc.titleAn International Proficiency Test to Detect, Identify and Quantify Ricin in Complex Matrices
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10042426
dc.identifier.doi10.3390/toxins7124859
dc.identifier.doihttp://dx.doi.org/10.25646/2145
local.edoc.container-titleToxins
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mdpi.com/2072-6651/7/12/4859
local.edoc.container-publisher-nameMDPI
local.edoc.container-volume7
local.edoc.container-issue12
local.edoc.container-year2015

Zur Kurzanzeige