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2015-11-26Zeitschriftenartikel DOI: 10.25646/2147
Qualitative and Quantitative Detection of Botulinum Neurotoxins from Complex Matrices: Results of the First International Proficiency Test
dc.contributor.authorWorbs, Sylvia
dc.contributor.authorFiebig, Uwe
dc.contributor.authorZeleny, Reinhard
dc.contributor.authorSchimmel, Heinz
dc.contributor.authorRummel, Andreas
dc.contributor.authorLuginbühl, Werner
dc.contributor.authorDorner, Brigitte
dc.date.accessioned2018-05-07T18:44:28Z
dc.date.available2018-05-07T18:44:28Z
dc.date.created2015-12-21
dc.date.issued2015-11-26none
dc.identifier.issn10.3390/toxins7124857
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reSVmsgeX4bLU/PDF/22zN7gYlccZTs.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2222
dc.description.abstractIn the framework of the EU project EQuATox, a first international proficiency test (PT) on the detection and quantification of botulinum neurotoxins (BoNT) was conducted. Sample materials included BoNT serotypes A, B and E spiked into buffer, milk, meat extract and serum. Different methods were applied by the participants combining different principles of detection, identification and quantification. Based on qualitative assays, 95% of all results reported were correct. Successful strategies for BoNT detection were based on a combination of complementary immunological, MS-based and functional methods or on suitable functional in vivo/in vitro approaches (mouse bioassay, hemidiaphragm assay and Endopep-MS assay). Quantification of BoNT/A, BoNT/B and BoNT/E was performed by 48% of participating laboratories. It turned out that precise quantification of BoNT was difficult, resulting in a substantial scatter of quantitative data. This was especially true for results obtained by the mouse bioassay which is currently considered as “gold standard” for BoNT detection. The results clearly demonstrate the urgent need for certified BoNT reference materials and the development of methods replacing animal testing. In this context, the BoNT PT provided the valuable information that both the Endopep-MS assay and the hemidiaphragm assay delivered quantitative results superior to the mouse bioassay.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectproficiency testeng
dc.subjectreference materialeng
dc.subjectstandardized detectioneng
dc.subjectBoNTeng
dc.subject.ddc610 Medizin
dc.titleQualitative and Quantitative Detection of Botulinum Neurotoxins from Complex Matrices: Results of the First International Proficiency Test
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10042442
dc.identifier.doihttp://dx.doi.org/10.25646/2147
local.edoc.container-titleToxins
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mdpi.com/2072-6651/7/12/4857
local.edoc.container-publisher-nameMDPI
local.edoc.container-volume7
local.edoc.container-issue12
local.edoc.container-year2015

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