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2016-03-17Zeitschriftenartikel DOI: 10.3389/fmicb.2016.00336
Carriage of Extended-Spectrum Beta-Lactamase-Plasmids Does Not Reduce Fitness but Enhances Virulence in Some Strains of Pandemic E. coli Lineages
dc.contributor.authorSchaufler, Katharina
dc.contributor.authorSemmler, Torsten
dc.contributor.authorPickard, Derek J.
dc.contributor.authorToro, María de
dc.contributor.authorCruz, Fernando de la
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorEwers, Christa
dc.contributor.authorGuenther, Sebastian
dc.date.accessioned2018-05-07T18:57:04Z
dc.date.available2018-05-07T18:57:04Z
dc.date.created2016-03-17
dc.date.issued2016-03-17none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reMavbYZsUZWo/PDF/20uNJPG1WUCw6.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2291
dc.description.abstractPathogenic ESBL-producing E. coli lineages occur frequently worldwide, not only in a human health context but in animals and the environment, also in settings with low antimicrobial pressures. This study investigated the fitness costs of ESBL-plasmids and their influence on chromosomally encoded features associated with virulence, such as those involved in the planktonic and sessile behaviors of ST131 and ST648 E. coli. ESBL-plasmid-carrying wild-type E. coli strains, their corresponding ESBL-plasmid-“cured” variants (PCV), and complementary ESBL-carrying transformants were comparatively analyzed using growth curves, Omnilog® phenotype microarray (PM) assays, macrocolony and biofilm formation, swimming motility, and RNA sequence analysis. Growth curves and PM results pointed toward similar growth and metabolic behaviors among the strains. Phenotypic differences in some strains were detected, including enhanced curli fimbriae and/or cellulose production as well as a reduced swimming capacity of some ESBL-carrying strains, as compared to their respective PCVs. RNA sequencing mostly confirmed the phenotypic results, suggesting that the chromosomally encoded csgD pathway is a key factor involved. These results contradict the hypothesis that ESBL-plasmid-carriage leads to a fitness loss in ESBL-carrying strains. Instead, the results indicate an influence of some ESBL-plasmids on chromosomally encoded features associated with virulence in some E. coli strains. In conclusion, apart from antibiotic resistance selective advantages, ESBL-plasmid-carriage may also lead to enhanced virulence or adaption to specific habitats in some strains of pandemic ESBL-producing E. coli lineages.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subject.ddc610 Medizin
dc.titleCarriage of Extended-Spectrum Beta-Lactamase-Plasmids Does Not Reduce Fitness but Enhances Virulence in Some Strains of Pandemic E. coli Lineages
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10043569
dc.identifier.doi10.3389/fmicb.2016.00336
dc.identifier.doihttp://dx.doi.org/10.25646/2216
local.edoc.container-titleFrontiers in Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.00336/full
local.edoc.container-publisher-nameFrontiers Media
local.edoc.container-volume7
local.edoc.container-issue336
local.edoc.container-year2016

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