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2016-06-17Zeitschriftenartikel DOI: 10.1038/srep28063
Comparison of six commercial kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing
dc.contributor.authorBecker, Laura
dc.contributor.authorSteglich, Matthias
dc.contributor.authorFuchs, Stephan
dc.contributor.authorWerner, Guido
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T19:07:24Z
dc.date.available2018-05-07T19:07:24Z
dc.date.created2016-07-01
dc.date.issued2016-06-17none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re3YG4mwEVVmM/PDF/244eNVc4orvSU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2348
dc.description.abstractWe compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of extraction kit had little effect on sequencing quality and coverage across drastically different replicons, except for an apparent depletion of small plasmids (eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subject.ddc610 Medizin
dc.titleComparison of six commercial kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10045680
dc.identifier.doi10.1038/srep28063
dc.identifier.doihttp://dx.doi.org/10.25646/2273
local.edoc.container-titleScientific Reports
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.nature.com/articles/srep28063
local.edoc.container-publisher-nameNature Publishing Group
local.edoc.container-volume6
local.edoc.container-issue28063
local.edoc.container-year2016

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