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2016-09-29Zeitschriftenartikel DOI: 10.1371/journal.pntd.0004953
A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
dc.contributor.authorPatel, Pranav
dc.contributor.authorWahed, Ahmed Abd El
dc.contributor.authorFaye, Oumar
dc.contributor.authorPrüger, Pauline
dc.contributor.authorKaiser, Marco
dc.contributor.authorThaloengsok, Sasikanya
dc.contributor.authorUbol, Sukathida
dc.contributor.authorSakuntabhai, Anavaj
dc.contributor.authorLeparc-Goffart, Isabelle
dc.contributor.authorHufert, Frank T.
dc.contributor.authorSall, Amadou A.
dc.contributor.authorWeidmann, Manfred
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2018-05-07T19:24:08Z
dc.date.available2018-05-07T19:24:08Z
dc.date.created2016-10-06
dc.date.issued2016-09-29none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/repVmgu0lcrkI/PDF/216CzUaXXZra2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2439
dc.description.abstractBackground: Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. Methodology/Principal Findings: In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. Conclusions/Significance: The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subject.ddc610 Medizin
dc.titleA Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10047169
dc.identifier.doi10.1371/journal.pntd.0004953
dc.identifier.doihttp://dx.doi.org/10.25646/2364
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004953
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume10
local.edoc.container-issue9
local.edoc.container-year2016

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