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2017-07-12Zeitschriftenartikel DOI: 10.1186/s12917-017-1140-2
Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions
dc.contributor.authorKoehler, Susanne M.
dc.contributor.authorBuyuk, Fatih
dc.contributor.authorCelebi, Ozgur
dc.contributor.authorDemiraslan, Hayati
dc.contributor.authorDoganay, Mehmet
dc.contributor.authorSahin, Mitat
dc.contributor.authorMoehring, Jens
dc.contributor.authorNdumnego, Okechukwu C.
dc.contributor.authorOtlu, Salih
dc.contributor.authorHeerden, Henriette van
dc.contributor.authorBeyer, Wolfgang
dc.date.accessioned2018-05-07T20:20:37Z
dc.date.available2018-05-07T20:20:37Z
dc.date.created2017-08-07
dc.date.issued2017-07-12none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/refnVbF4Yo21U/PDF/29x6gGaAfLk6g.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2744
dc.description.abstractBackground: Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne live spore vaccine (34F2) in animals. However, live spore vaccines are not suitable for simultaneous vaccination and antibiotic treatment of animals being at risk of infection in an outbreak situation. Non-living vaccines could close this gap. Results: In this study a combination of recombinant protective antigen and recombinant Bacillus collagen-like antigen (rBclA) with or without formalin inactivated spores (FIS), targeted at raising an immune response against both the toxins and the spore of B. anthracis, was tested for immunogenicity and protectiveness in goats. Two groups of goats received from local farmers of the Kars region of Turkey were immunized thrice in three weeks intervals and challenged together with non-vaccinated controls with virulent B. anthracis, four weeks after last immunization. In spite of low or none measurable toxin neutralizing antibodies and a surprisingly low immune response to the rBclA, 80% of the goats receiving the complete vaccine were protected against a lethal challenge. Moreover, the course of antibody responses indicates that a two-step vaccination schedule could be sufficient for protection. Conclusion: The combination of recombinant protein antigens and FIS induces a protective immune response in goats. The non-living nature of this vaccine would allow for a concomitant antibiotic treatment and vaccination procedure. Further studies should clarify how this vaccine candidate performs in a post infection scenario controlled by antibiotics.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectVaccinationeng
dc.subjectAnthraxeng
dc.subjectBacillus anthraciseng
dc.subjectAnimal vaccineeng
dc.subject.ddc610 Medizin
dc.titleProtection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10054200
dc.identifier.doi10.1186/s12917-017-1140-2
dc.identifier.doihttp://dx.doi.org/10.25646/2669
local.edoc.container-titleBMC Veterinary Research
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1140-2
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume13
local.edoc.container-issue220
local.edoc.container-year2017

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