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2017-08-15Zeitschriftenartikel DOI: 10.1128/mBio.01070-17
Comparative Genomics of Escherichia coli Isolated from Skin and Soft Tissue and Other Extraintestinal Infections
dc.contributor.authorRanjan, Amit
dc.contributor.authorShaik, Sabiha
dc.contributor.authorNandanwar, Nishant
dc.contributor.authorHussain, Arif
dc.contributor.authorTiwari, Sumeet K.
dc.contributor.authorSemmler, Torsten
dc.contributor.authorJadhav, Savita
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorAlam, Munirul
dc.contributor.authorColwell, Rita R.
dc.contributor.authorAhmed, Niyaz
dc.date.accessioned2018-05-07T20:22:19Z
dc.date.available2018-05-07T20:22:19Z
dc.date.created2017-08-16
dc.date.issued2017-08-15none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reZwXdun7g05Y/PDF/26lff69FzEfV2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2753
dc.description.abstractEscherichia coli, an intestinal Gram-negative bacterium, has been shown to be associated with a variety of diseases in addition to intestinal infections, such as urinary tract infections (UTIs), meningitis in neonates, septicemia, skin and soft tissue infections (SSTIs), and colisepticemia. Thus, for nonintestinal infections, it is categorized as extraintestinal pathogenic E. coli (ExPEC). It is also an opportunistic pathogen, causing cross infections, notably as an agent of zoonotic diseases. However, comparative genomic data providing functional and genetic coordinates for ExPEC strains associated with these different types of infections have not proven conclusive. In the study reported here, ExPEC E. coli isolated from SSTIs was characterized, including virulence and drug resistance profiles, and compared with isolates from patients suffering either pyelonephritis or septicemia. Results revealed that the majority of the isolates belonged to two pathogenic phylogroups, B2 and D. Approximately 67% of the isolates were multidrug resistant (MDR), with 85% producing extended-spectrum beta-lactamase (ESBL) and 6% producing metallo-beta-lactamase (MBL). The blaCTX-M-15 genotype was observed in at least 70% of the E. coli isolates in each category, conferring resistance to an extended range of beta-lactam antibiotics. Whole-genome sequencing and comparative genomics of the ExPEC isolates revealed that two of the four isolates from SSTIs, NA633 and NA643, belong to pandemic sequence type ST131, whereas functional characteristics of three of the ExPEC pathotypes revealed that they had equal capabilities to form biofilm and were resistant to human serum. Overall, the isolates from a variety of ExPEC infections demonstrated similar resistomes and virulomes and did not display any disease-specific functional or genetic coordinates.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subject.ddc610 Medizin
dc.titleComparative Genomics of Escherichia coli Isolated from Skin and Soft Tissue and Other Extraintestinal Infections
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10054285
dc.identifier.doi10.1128/mBio.01070-17
dc.identifier.doihttp://dx.doi.org/10.25646/2678
local.edoc.container-titlemBio
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://mbio.asm.org/content/8/4/e01070-17
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume8
local.edoc.container-issue4
local.edoc.container-year2017

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