Show simple item record

2017-09-05Zeitschriftenartikel DOI: 10.1186/s12864-017-4095-6
Dual RNA-seq reveals no plastic transcriptional response of the coccidian parasite Eimeria falciformis to host immune defenses
dc.contributor.authorEhret, Totta
dc.contributor.authorSpork, Simone
dc.contributor.authorDieterich, Christoph
dc.contributor.authorLucius, Richard
dc.contributor.authorHeitlinger, Emanuel
dc.date.accessioned2018-05-07T20:37:20Z
dc.date.available2018-05-07T20:37:20Z
dc.date.created2017-10-04
dc.date.issued2017-09-05none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rekHhzDpRVwww/PDF/23DnZs6M3oaMY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2834
dc.description.abstractBackground: Parasites can either respond to differences in immune defenses that exist between individual hosts plastically or, alternatively, follow a genetically canalized (“hard wired”) program of infection. Assuming that large-scale functional plasticity would be discernible in the parasite transcriptome we have performed a dual RNA-seq study of the lifecycle of Eimeria falciformis using infected mice with different immune status as models for coccidian infections. Results: We compared parasite and host transcriptomes (dual transcriptome) between naïve and challenge infected mice, as well as between immune competent and immune deficient ones. Mice with different immune competence show transcriptional differences as well as differences in parasite reproduction (oocyst shedding). Broad gene categories represented by differently abundant host genes indicate enrichments for immune reaction and tissue repair functions. More specifically, TGF-beta, EGF, TNF and IL-1 and IL-6 are examples of functional annotations represented differently depending on host immune status. Much in contrast, parasite transcriptomes were neither different between Coccidia isolated from immune competent and immune deficient mice, nor between those harvested from naïve and challenge infected mice. Instead, parasite transcriptomes have distinct profiles early and late in infection, characterized largely by biosynthesis or motility associated functional gene groups, respectively. Extracellular sporozoite and oocyst stages showed distinct transcriptional profiles and sporozoite transcriptomes were found enriched for species specific genes and likely pathogenicity factors. Conclusion: We propose that the niche and host-specific parasite E. falciformis uses a genetically canalized program of infection. This program is likely fixed in an evolutionary process rather than employing phenotypic plasticity to interact with its host. This in turn might limit the potential of the parasite to adapt to new host species or niches, forcing it to coevolve with its host.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectPhenotypic plasticityger
dc.subjectParasite lifecycleger
dc.subjectTranscriptional plasticityger
dc.subjectApicomplexager
dc.subjectDual RNA-seqger
dc.subjectDual transcriptomicsger
dc.subjectCoccidiager
dc.subject.ddc610 Medizin
dc.titleDual RNA-seq reveals no plastic transcriptional response of the coccidian parasite Eimeria falciformis to host immune defenses
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10055190
dc.identifier.doi10.1186/s12864-017-4095-6
dc.identifier.doihttp://dx.doi.org/10.25646/2759
local.edoc.container-titleBMC Genomics
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4095-6
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume18
local.edoc.container-issue686
local.edoc.container-year2017

Show simple item record